【摘要】 目的　评价氧化铁纳米颗粒 (IONP)作为体外基因载体的可行性及其外加磁场对于其转染效率的影响。方法　以碱沉淀法制成IONP ,在其表面修饰多聚赖氨酸制成多聚赖氨酸 氧化铁纳米颗粒 (IONP PLL)。应用荧光素酶报告基因系统 ,检测IONP PLL将外源基因转染至不同的细胞系的转染特性 ,同时 ,应用钕 铁 硼稀土强磁场结合荧光素酶报告基因 ,评价外加磁场对于IONP PLL作为基因载体效率的影响。结果　IONP可将外源基因转染至多个细胞系并高效表达。不同细胞系的转染效率和时间各不相同。外加磁场可使转染效率提高 5～ 10倍。结论　磁性IONP是一种可用于体外转染的新型非病毒基因载体 ,外加磁场可提高其转染效率。更多还原

【Abstract】 Objective To evaluate the feasibility of using iron oxide nanoparticles as gene vector and the effect of magnetic field on efficiency of transfection. Methods Iron oxide nanoparticles were prepared by alkaline precipitation of divalent and trivalent iron chloride. The surface of iron oxide nanoparticles was modified by self-assembled poly-L-lysine to form particle complexes (IONP-PLL). Transfection was determined by delivering reporter gene, PGL2-control encoding luciferase, to different cell lines using IONP-PLL as vector. The effect of magnetic field on efficiency of transfection was determined using Nd-Fe-B permanent magnet. Results Foreign gene could be delivered to various cell lines by IONP-PLL and expressed with high efficiency, but the transfection efficiency and time course varied in the different cell lines studied. Magnetic field could enhance the efficiency of transfection by 5～10 fold. Conclusion IONP-PLL can be used as a novel non-viral gene vector in vitro, which offers a basis for gene delivery in vivo.更多还原