【摘要】 目的　检测黑色素瘤抗原MAGE B基因 (MAGE B1,MAGE B2 )在肝细胞癌 (HCC)中的表达 ,以期找到可用于HCC免疫治疗的新靶位。方法　用逆转录 聚合酶链反应 (RT PCR)方法检测MAGE B1、B2、A1和A3在 47例HCC患者癌组织和相应癌旁组织、3 0例肝硬化和正常肝组织中的表达 ,随机选择 4例RT PCR阳性扩增产物直接进行DNA序列测定 ,并将其表达结果与临床指标之间的关系进行分析。结果　 47例HCC组织中 ,MAGE B1和MAGE B2基因阳性率分别为 44.7% (2 1/47)和61.7% (2 9/47) ,而相应癌旁组织、肝硬化组织和正常肝组织中均为阴性。测序结果证实 ,RT PCR产物确为这两种基因。MAGE A1和MAGE A3的阳性率分别为 74.5% (3 5/47)和 44.7% (2 1/47)。MAGE B和MAGE A的表达之间有显著相关性 (P <0 .0 5) ,但其中 12例不表达MAGE A1和 (或 )A3的病例中 ,有 5例表达MAGE B1和 (或 )B2。同时检测这 4种基因的表达 ,发现至少表达其中 1种、2种、3种和4种均表达者分别为 83 .0 % (3 9/47)、55.3 % (2 6/47)、48.9% (2 3 /47)和 3 8.3 % (18/47)。MAGE B基因的表达与年龄、性别、肿瘤大小、分化程度、血清甲胎球蛋白 (AFP)水平、HBV和HCV感染之间无显著相关性 (P >0 .0 5)。结论　MAGE B基因家族在HCC中呈相对高比例、高特异表达 ,可更多还原

【Abstract】 Objective To investigate the expression of MAGE-B genes in hepato cellular carcinoma(HCC) in order to find new targets for immunotherapy. Methods The expression of MAGE-B1, B2, A1 and A3 mRNA was detected using RT-PCR in HCC tissues and the corresponding adjacent non-HCC tissues from 47 HCC pati e nts , 30 samples of cirrhosis and normal liver tissues. Four samples selected ra ndomly from MAGE-B1 or B2 with positive RT-PCR results were sequenced to confi rm the results of RT-PCR. The relationship between the expression of MAGE-B and some clinicopathological parameters was analyzed. Results MAGE-B1 mRNA and MAGE-B2 mRNA were detected in 44.7%(21/47)and 61.7%(29/47)of HCC samples, respectiv ely, while neither MAGE-B1 nor MAGE-B2 could be detected in the corresponding ad jacent non-HCC liver tissues. In addition, none of 30 samples of cirrhosis and n ormal liver tissues was shown to express both MAGE-B genes. The DNA sequence co n firmed that the RT-PCR products were truly target cDNA. The frequency of the ex p ression of MAGE-A1 and A3 was 74.5%(35/47) and 44.7%(21/47), respectively. Ther e was significant correlation between the expression of MAGE-B and MAGE-A( P <0.05 ). However, the positive expression of MAGE-B was observed in 5 out of 12 HCC t issues without expression of MAGE-A1 and/or A3. When all four MAGE genes were e xamined, the positive rate of expression of one, two, three and four genes was 8 3.0%(39/47), 55.3%(26/47), 48.9%(23/47), and 38.3%(18/47) of 47 HCC tissues, r esp ectively. No correlation was found between the expression of MAGE-B and clinica l parameters such as age, sex, tumor size, degree of tumor differentiation, serum α-fetoprotein level and hepatitis B virus or hepatitis C virus infection ( P >0.05). Conclusion MAGE-B genes are expressed with relatively high frequency and specificity in HCC. Most HCC patients with positive expression of at least one m ember of MAGE-B or MAGE-A gene family are adequate candidates to receive speci fic immun otherapy. Frequent co-expression of multiple members of MAGE-B and MAGE-A subfam ilies provides the possibility of using polyvalent vaccines to achieve more effe ctive immunotherapeutic results.更多还原