【摘要】 目的 探讨转化生长因子β1(TGF-β1)对大鼠结缔组织生长因子(CTGF)基因启动子活性的调控作用。方法 将大鼠CTGF基因启动子片段与荧光素酶报告基因组成重组体pCTGF-luc。采用脂质体转染方法将该质粒转至原代分离并培养的2～5代大鼠胰腺星状细胞(PSCs)中,24 h后分别在不同时间和不同浓度的体外TGF-β1刺激下用Dual-luciferase报告系统检测相应荧光素酶的活性。结果 TGF-β1表现为以剂量和时间依赖的方式上调PSCs中CTGF基因启动子活性,TGF-β1在短时间内即可使CTGF基因启动子活性明显增高,并达到高峰,且能有效地保持至36 h左右。结论 在PSCs中,TGF-β1主要在转录水平调节CTGF表达,较小浓度和较短时间即可使CTGF启动子活性增强。更多还原

【Abstract】 Objective To investigate the molecular mechanism for transforming growth factor β1 (TGF-β1)on regulation of connective tissue growth factor(CTGF) gene promoter in pancreatic stellate cells ( PSCs) . Methods We tried to transfect the passaged 2 - 5 PSCs with pCTGF-luc plasmids, which were composed of CTGF promoter and PGL3 vector. And different concentrations of TGF-β1 or stimulation time were used to observe the reaction of luciferase activities by the measurement of dual-luciferase assay system. Results TGF-β1 could enhance the activities of pCTGF-luc in PSCs by means of time and dose dependently. TGF-β1 could stimulate the expression of CTGF gene promoter at the high level in a short time. Conclusion TGF-β1 could enhance the activities of CTGF promoter in PSCs.更多还原