【摘要】 目的　探讨内皮抑素蛋白抑制激光诱导的大鼠脉络膜新生血管 (CNV)的效果。方法采用基因重组方法获得内皮抑素蛋白 ,取BrownNorway大鼠 30只 (6 0只眼 ) ,用激光光凝方式建立CNV模型 ,随机分为内皮抑素组、生理盐水组及对照组 ,每组 10只 ,分别给予内皮抑素 2 0 μl(5g/L) ,生理盐水 2 0 μl视网膜下注射 ,对照组光凝后不做处理 ,观察期为 14d。应用激光共焦显微镜下脉络膜血管平铺法测量各组CNV面积 ,以荧光素眼底血管造影后荧光素渗漏程度、光镜下观察结果、CD10 5及因子Ⅷ免疫组化染色结果作为观察指标。结果　内皮抑素组CNV面积小于生理盐水组及对照组 (F=30 7 35 1,P <0 0 0 1) ;内皮抑素组各光凝斑荧光渗漏程度评分与生理盐水组及对照组间比较 ,差异有显著意义 (χ2 =13 711,P <0 0 0 1) ;光凝后 14d ,生理盐水组和对照组光凝区脉络膜层增厚 ,脉络膜毛细血管层内皮细胞增殖 ,新生血管增生 ,内皮抑素组内皮细胞增生数量及新生血管明显少于前两组 ;CD10 5、因子Ⅷ的阳性表达量明显降低 ;各组视网膜内核层及神经纤维层内皮细胞均无CD10 5阳性表达。结论　内皮抑素可以有效抑制CNV的形成 ,脉络膜血管平铺及CD10 5检测对于CNV的定性、定量评估具有一定意义。更多还原

【Abstract】 Objective Endostatin is an endogeneous angiogenesis inhibitor. The purpose of this study was to investigated the effect of Endostatin on the eyes of rats with experimential choroidal neovascularization (CNV). Methods Experimental CNV was induced by laser photocoagulation. Animals were given subretinal injections of recombinant human Endostatin 20 μl(5 g/L) or 0.9% chlorine sodium . The intensity of fluorescein leakage from the photocoagulated lesions was studied 13days after photocoagulation. The area of CNV at each rupture site was measured using high molecular weight FITC-dextran (MW 2×10 6)for high resolution angiography in RPE-choroid-sclera flat mounts.In addition,8 eyes in each group were removed and fixed 14 days after photocoagulation,cut into thin sections. The sections were examined by light microscopy. Immunolocalization of Endoglin(CD105) and factor Ⅷ on sections of CNV lesions was studied by immunohistochemical evaluation. Results After Endostatin injection,fluorescein leakage from the CNV lesions decreased significantly compared with the control eyes. The average area of CNV at sites of the Bruch′s membrane rupture showed significant difference in eyes injected with Endostatin compared with control eyes .Endothelial cells demonstrated strong immunoreactivity of CD105 and factor Ⅷ in CNV lesions of control eyes. CD105-positive cell were not detected in normal chorioretinal tissues. Conclusions The development of CNV can be inhibited by injection of Endostatin,which suggest that Endostatin may be benefical in treating CNV and that further studies can be considered to evaluate this possibility.(Chin J Ophthalmol,2004,40:266-271)更多还原