【摘要】 目的　研究噪声磁场对低强度工频磁场诱导或增强致癌物 12 氧 14 酰佛波 13酯(TPA)对细胞缝隙连接通讯功能 (GJIC)抑制作用的干预。方法　NIH3T3小鼠成纤维细胞分别受5 0Hz 0 2mT、0 2mT +TPA极低频磁场或 (和 )同等强度的噪声磁场作用 2 4h后 ,采用激光共聚焦显微镜 ,用荧光漂白后恢复技术测定细胞的GJIC功能。结果　 0 2mT工频磁场与TPA可协同抑制GJIC功能 ,其荧光恢复率为 (2 3± 11) % ,与对照组的 (46± 19) %及TPA组的 (34± 17) %比较 ,均差异有非常显著性 ;当叠加 0 2mT噪声磁场后 ,荧光恢复率为 (35± 19) % ,可显著拮抗 0 2mT磁场对TPA的协同抑制作用。结论　 0 2mT噪声磁场可以阻断同等强度磁场协同TPA抑制细胞GJIC的作用。更多还原

【Abstract】 Objectives To explore intervention with electromagnetic noise for co-suppression effect on gap-junctional intercellular communication (GJIC) induced or strengthened by low intensity magnetic field with carcinogen 12-O-tetradecanoylphorbol-13-acetate (TPA). Methods Fibroblast cells from NIH 3T3 mice were exposed to extremely low intensity magnetic field (MF) 0.2 mT, 0.2 mT+TPA or/and electromagnetic noise with the same intensity of MF for 24h, and GJIC was determined using fluorescence recovery analysis after photobleaching (FRAP) with a laser-scanning confocal microscope (Leica, Germany). Results GJIC function could be co-suppressed by MF of 0.2 mT with TPA, with fluorescence recovery of (23±11)%, lower than that in the control group [(46±19)%] and in the group with TPA only [(34±17) %] ( P <0.01), indicating 0.2 mT MF plus TPA could co-inhibit GJIC( P <0.01). Superposition of 0.2 mT noise MF could get a fluorescence recovery of(35±19)% and significantly antagonize its co-suppression by TPA. Conclusion Electromagnetic noise of 0.2 mT could block the intensifying effect of power frequency magnetic field on TPA-induced GJIC inhibition.更多还原