【摘要】 目的研究T辅助细胞转录因子T bet在急性移植物抗宿主病 (aGVHD)发生过程中的作用 ,评估T bet与aGVHD的相关性。方法以逆转录 聚合酶链反应 (RT PCR)方法检测 2 0例异基因造血干细胞移植 (allo HSCT)患者预处理前、0、移植后 14 (+14 )、+2 8、+42天骨髓单个核细胞(MNC)T betmRNA的表达变化。结果allo HSCT患者出现aGVHD时 ,骨髓MNCT betmRNA的表达较出现前增高 ,治疗后降低 ;+14天 ,在T betmRNA的表达较预处理前增高的 12例患者中有11例 (91.7% )发生aGVHD ,表达未增高的 8例患者中有 1例 (12 .5 % )发生aGVHD。结论　allo HSCT后患者T betmRNA的表达较预处理前增高 ,发生aGVHD的可能性大。用定量或半定量RT PCR方法检测T betmRNA的表达有助于指导aGVHD的诊断和治疗情况的监测更多还原

【Abstract】 Objective To study the role of T-bet \[a T helper 1(Th1) lymphocyte transcription factor\] gene expression in predicting acute graft-versus-host disease(aGVHD) and evaluate the correlation between T-bet gene and aGVHD.Methods Twenty patients who underwent allogeneic stem cell transplantation(allo-HSCT) entered this study. The expression of T-bet gene was examined with reverse transcription(RT)-PCR in bone marrow samples collected from patients on the day before conditioning, and day 0, day 14, day 28, and day 42 after HSCT. ResultsThe expression level of T-bet in patients developed aGVHD was increased compared with that before conditioning (P=0.043). The incidence of aGVHD was 91.7% in patients whose T-bet expression level was increased on day 14 after transplant while was 12.5% in those whose T-bet gene expression level was not increased on that day (P<0.001).ConclusionPatients with increased expression levels of T-bet after allo-HSCT may have an increased possibility to develop aGVHD. T-bet expression level may serve as an advisable guide to the clinician in predicting aGVHD and monitoring treatment.更多还原