【摘要】 目的　研究小鼠脑组织同源框基因 (homeoboxgene ,Hox)的表达状态及人巨细胞病毒(humancytomegalovirus ,HCMV)感染对小鼠脑组织Hox基因表达的影响 ,以探讨HCMV致畸的分子机制。方法　昆明系小鼠 4 8只 ,随机分为 :实验对照组 (16只 )和病毒感染组 (32只 ) ,病毒感染为脑内注射。在感染后 7、15、30、6 0d分别以病理学方法观察病理损伤 ,以免疫组化方法检查HCMV LA(latean tigen) ,PCR检测小鼠脑组织中HCMV DNA。在建立HCMV脑部感染的小鼠模型基础上 ,用RT PCR测定Hox基因在病毒感染组和实验对照组小鼠脑组织中的表达 ,并用同位素标记的Hox寡核苷酸探针进行Northernblot检测相应的表达状况。结果　 (1)接种HCMVAD16 9株的小鼠脑组织发生了病理改变 ,免疫组化和PCR方法在神经细胞内查到了HCMV LA和DNA ;(2 )RT PCR和Northernblot发现 :对照组的小鼠脑组织表达Hoxa2、Hoxb1和Hoxb2 ,但不表达Hoxb5、Hoxb8;HCMV感染后 ,小鼠脑组织被诱导表达Hoxa1,与对照组比较差异有显著性意义 (P <0 .0 1) ,且于感染后的 15d达到高峰 ,而Hoxb1的表达下调 (P <0 .0 5 ) ;Hoxa2和Hoxb2无明显变化 ;Hoxb5和Hoxb8仍旧不表达。结论　HCMVAD16 9株可感染小鼠神经系统。HCMV感染可诱导小鼠神经系统发育基因Hox基因表达改变 ,这对更多还原

【Abstract】 ObjectiveTo study the expressions of Hox genes in murine brain and influence of human cytomegalovirus(HCMV) infection on the expressions of these genes. MethodsForty eight kunming mice were randomly divided into infection group (32) injected with HCMV AD169 and control group (16) injected with saline into their brain. After 7,15,30 and 60 days,the cerebral lesions were observed by pathological method,HCMV antigen was detected by immunohistochemical method and HCMV DNA was sequenced by polymerase chain reaction(PCR). On the basis of developing HCMV mouse model,reverse trancriptase-polymerase chain reaction(RT-PCR)was applied to determine the expressions of Hox genes in murine brain meanwhile,the expressions of Hox genes were analysed by Northern blot with isotope labelled Hox genes oligonucleotide probes. ResultsA HCMV infection model was developed and extensive pathological damages in brain tissue of infected mice were observed. Meanwhile,the HCMV-LA and HCMV-DNA were also found in brain tissues of HCMV infected mice. The expression level of Hox genes in control and infected mouse brain were determined by RT-PCR and Northern blot,RT-PCR and Northern blot showed that mouse brain expressed Hoxa2,Hoxb1 and Hoxb2 gene,but they did not expressed Hoxb5 and Hoxb8 gene. After HCMV infection,murine brain was induced to express Hoxa1 gene(P< 0.01),and reached the peak at 15 d after infection. Comparing with expression of control group,the expression of Hoxb1 was down-regulated in infection group(P<0.05). The expression of Hoxa2,Hoxb2 gene had no significant change,Hoxb5 and Hoxb8 genes were still not expressed. ConclusionThe results suggest that HCMV AD169 is able to cause mouse CNS infection and induce the abnormal expressions of Hox genes,which provides more information for understanding the mechanism of congenital abnormal due to HCMV infection and a valuable method of clinical prevention and treatment of HCMV infection. [更多还原