【摘要】 目的　研究稳定存活的肝移植受者血清细胞因子的表达状况及意义。方法　ELISA检测 2 2例原位肝移植患者、13例原发性肝癌患者及 12例正常人血清细胞因子的表达情况。流式细胞术分析外周血T细胞表型。结果　外周血CD3+ 、CD8+ T细胞百分比以及CD4 + /CD8+ 比值 ,各组间比较无统计学差异。肝移植组CD3+ CD2 5 + T细胞百分比高于正常人组 (P =0 0 2 2 )。血清IL 2、IFN γ、IL 10、IL 4及TNF α水平 ,各组间比较差异无显著性。IL 6、ICAM 1和P selectin水平肝移植组显著高于正常人组 (P值分别为 0 0 4 8、0 0 0 0和 0 0 2 5 )。结论　稳定存活的肝移植患者体内效应T细胞仍处于低活化状态 ,炎症性细胞因子 (TNF α、IL 6 )和黏附分子 (ICAM 1、P Selectin)可能在移植物的慢性损伤过程中发挥了作用更多还原

【Abstract】 Objective To elucidate the profile of serum cytokines and adhesion molecules in stable survivors with clinical liver transplantation Methods Flow cytometric analysis was used to analyse the phenotype of T cell subsets in peripheral blood mononuclear cells (PBMCs) from group of liver transplantation(LTx) ( n =22),primary liver carcinoma (PLC)( n =13) and healthy control ( n =12) Enzyme linked immunoabsorbent assay (ELISA) was used to determine the serum cytokines and adhesion molecules profiles in stable survivors with clinical liver transplantation Results Percentage of CD3 + T cell and CD8 + T cell,as well as ratio of CD4 + to CD8 + revealed no difference among three groups The percentage of CD3 + CD25 + T cells in LTx group was found higher than that in healthy group ( P =0 022) Th1 cytokines( IL 2,IFN γ) and Th2 cytokines( IL 4,IL 10),as well as TNF α displayed no significant difference among three groups The levels of IL 6, ICAM 1 and P selectin in serum were not found any difference between LTx group and PLC group ,while the levels of IL 6,ICAM 1 and P selectin in serum shown significant difference between LTx and healthy groups ( P =0 048、0 000 and 0 025,respectively) Conclusions Our data demonstrates that effector T cells can also be activated and exert immunoresponse to grafts permanently under the treatment of immunosuppressant Adhesion molecules (ICAM 1,P Selectin) and pro inflammatory cytokines(IL 6,TNF α) might be involved in the process of chronic graft damage induced by allo immunoresponse更多还原