【摘要】 目的　探讨肢体缺血因素对骨髓内皮祖细胞 (EPC)的动员及肢体血管新生的影响。方法　切除、结扎单侧股总、股浅动脉建立大鼠后肢缺血模型 ,另设对照组。 1周后通过细胞培养 ,观察外周血EPC数量的变化 ,4周后检测缺血组织微血管密度 (MVD )及血管内皮生长因子(VEGF)的表达情况。结果　实验组外周血EPC (4 8.6± 7.5 )个 /mm2 较对照组 (2 3 .1± 4.3 )个 /mm2 明显增加 (P <0 .0 1) ;组织MVD (2 2 4.84± 5 .87)n/mm2 显著高于对照组 (13 6.3 0± 4.5 2 )n/mm2 (P <0 .0 1) ;实验组缺血组织VEGF表达明显增强 (吸光度值A :0 .162± 0 .0 18) ,对照组仅少量表达 (A :0 .0 5 5± 0 .0 0 6) ;外周血EPC与组织MVD、VEGF吸光度之间有非常显著相关 (P <0 .0 1)。结论　肢体缺血通过动员骨髓EPC ,促进缺血组织的新血管生成 ,改善局部供血 ,达到部分代偿目的。更多还原

【Abstract】 Objective To explore the effect of limb ischemia on mobilization of bone marrow derived endothelial progenitor cells (EPCs) and neovascularization of ischemic tissue.Methods Unilateral ischemic model of the hindlimbs was produced by excision of the common and superficial femoral artery in 20 male rats.The other 20 animals served as the control group.At week 1 after operation,the change of the circulating EPCs was observed by mononuclear cells culture.Microvessel density and the VEGF expression of ischemic limbs was detected at week 4.Results The number of circulating EPCs in experiment group was significantly increased as compared with the control group (48.6± 7.5/ mm 2 vs 23.1± 4.3/ mm 2, P < 0.01). Microvessel density of ischemic limbs was significantly higher than that in control group (224.84± 5.87/ mm 2 vs 136.30± 4.52/ mm 2, P < 0.01). The VEGF expression in experiment group was significantly higher than that in control group ( A : 0.162± 0.018 vs 0.055± 0.006, P < 0.01). The number of circulating EPCs was greatly related to the microvessel density and VEGF expression ( P < 0.01). Conclusion Limb ischemia can augment local perfusion by improving neovascularization with EPCs mobilization.更多还原