【摘要】 目的 观察七氟醚吸入麻醉不同时期大鼠各脑区Na⁺、K⁺-ATP酶和Ca²⁺-ATP酶活性动态变化规律,以了解脑ATP酶活性变化在七氟醚麻醉效应中的地位。方法 40只SD大鼠随机均分为对照组、诱导期组、麻醉期组、恢复期组和清醒期组。分光光度法测定不同时期大脑皮质、脑干、海马Na⁺、K⁺-ATP酶和Ca²⁺-ATP酶活性变化。结果 与对照组比较,大鼠大脑皮质、脑干、海马Na⁺、K⁺-ATP酶活性在诱导期分别降低21.9％、10.5％、19.3％（P<0.05）,麻醉期分别降低37.2％、33.5％、38.9％（P均<0.01）,恢复期又开始回升,但仍低于对照组水平（P<0.05或P<0.01）,而清醒期基本恢复至对照组水平（P>0.05）;大脑皮质、脑干、海马Ca²⁺-ATP酶活性在诱导期分别降低34.3％、44.3％、25.5％（P<0.05 or P<0.01）,麻醉期分别降低39.6％、60.4％、57.6％（P均<0.01）,恢复期开始回升,清醒期基本恢复至对照组水平（P>0.05）。结论七氟醚对大鼠大脑皮质、脑干、海马Na⁺、K⁺-ATP酶和Ca²⁺-ATP酶活性的抑制程度与麻醉时相相对应,ATP酶活性与麻醉深度具有相同的变化趋势,表明上述脑区ATP酶可能是七氟醚的作用靶点,与七氟醚的麻醉效应有关。更多还原

【Abstract】 Objective To observe the changes of Na⁺, K⁺ -ATPase and Ca²⁺-ATPase activity in different brain regions of rat at different anesthesia stages duringsevoflurane anesthesia and explore the role of ATPase in the anesthetic mechanism of sevoflurane. Methods Forty rats weighing 250-300 g were randomly divided into five groups of 8 animals each, control, induction of anesthesia, maintenance of anesthesia, recovery from anesthesia and complete recovery. Rats were placed in a special glass anesthesia box and exposed to 2.5% sevoflurane. The induction of anesthesia started from staggering of the animal to loss of righting reflex. The maintenance of anesthesia was from 1min after loss of righting reflex. The recovery of anesthesia lasted from recovery of righting reflex to staggering after sevoflurane anesthesia was discontinued and glass box was opened and the complete recovery was defined from 1h after recovery of righting reflex. The animals were decapitated at different stages of anesthesia. Cerebral cortex, hippocapus and brain stem were immediately removed on ice and frozen in liquid nitrogen. The Na⁺, K⁺-ATPase and Ca²⁺-ATPase activity in different brain regions of rat at different anesthesia stages were measured by spectrophotometer. Results The Na⁺ , K⁺-ATPase activity in cerebral cortex, brain stem and hippocampus begun to decrease during the induction of anesthesia and reached the lowest level during maintenance of anesthesia （P<0.01 ）, begun to increase during recovery from anesthesia and returned to preanesthetic level during complete recovery （P>0.05）. The Ca²⁺-ATPase activity in different brain regions begun to decrease during the induction of anesthesia （P< 0.05 or P< 0.01 ） and reached the lowest level during maintenance of anesthesia （P < 0.01 ）, begun to increase during recovery from anesthesiaand returned to the normal level during complete recovery （P > 0.05 ）. Conclusion Sevoflurane significantly inhibits the Na⁺, K⁺ -ATPase and Ca²⁺ -ATPase activity inthe brain, which is closely related to the depth of anesthesia, which suggest that Na⁺, K⁺ -ATPase and Ca²⁺ -ATPase in central nervous system may be pharmacologictargets for sevoflurane and be related to the mechanism of sevoflurane anesthesia.更多还原