【摘要】 目的　检测HBV DNA阅读框架各位点的变异 ,为临床诊断和治疗提供依据。方法　通过基因芯片技术 ,将HBV DNA进行PCR扩增 ,掺入荧光分子标记 ,与点阵列的寡核苷酸杂交 ,通过计算机分析 ,检测HBV DNA的基因序列 ,观察HBV DNA阅读框架各位点的自然变异。结果　HBV DNA阅读框架各位点的变异普遍存在 ,其中前C区 1896、1814变异率分别为 2 3 5 %、3 9% ,BCP 176 2、176 4变异率分别为 5 5 9%、5 3 9% ,P区 5 2 8、5 5 2M I、5 5 2M V变异率分别为 39 2 %、38 2 %、10 8%。结论　基因芯片技术是检测基因突变、基因测序的高效方法之一 ,具有极高的灵敏性和可靠性。HBV DNA位点的变异对于疾病的稳定与进展以及病情转归的预判有重要作用更多还原

【Abstract】 Objective To detect the mutation of HBV-DNA open reading frame (ORF),provide evidences for clinical diagnosis and treatment. Methods By using gene chip technique, HBV-DNA was amplified by PCR, incorporated with fluorescence, hybridized with oligonucleotide to detect the gene sequence of DNA by computer analysis and observe the natural mutation of HBV-DNA ORF. Results The mutations of HBV-DNA ORF existed widespread.The rates of mutation on Pre-C 1896,PreC 1814,BCP 1762,BCP 1764,P 528,P 552MI,P 552MV were 23.5%,3.9%,55.9%,53.9%,39.2%,38.2%,10.8%, respectively. Conclusion The gene chip technique posseses extremely high sensitivity and reliability,it is one of the effective methods to detect gene mutation.The mutation of HBV-DNA has important influence on the stability and progress of the disease, and on the judgement of prognosis.更多还原