【摘要】 目的研究加味桃红四物汤对角质形成细胞(KC)分泌内皮素-1和碱性成纤维细胞生长因子的影响。方法以体外培养的人角质形成细胞为对象,加入不同浓度加味桃红四物汤后,用MTT法反映细胞增殖的变化。ELISA方法测定细胞上清液中内皮素-1和碱性成纤维细胞生长因子的分泌量。结果加味桃红四物汤浓度4.27、5.49、6.1mg/mL时可促进KC的增殖。加味桃红四物汤浓度1.83mg/mL和5.49mg/mL可刺激KC分泌内皮素-1,而对KC分泌碱性成纤维细胞生长因子无影响。结论高浓度的加味桃红四物汤可促进KC的增殖。不同浓度的加味桃红四物汤均能刺激KC分泌内皮素-1,提示加味桃红四物汤在体外可刺激KC分泌内皮素-1。更多还原

【Abstract】 Objective To investigate the effect of ingredients-added Taohongsiwutang(a decoction of medicinal herbs, the decoction) on the secretion of endothelin-1 and basic fibroblast growth factor of kera-tinocytes. Methods Human keratinocytes were cultured in vitro. The viability and proliferation of kera-tinocytes treated with different concentrations of the decoction were assessed by MTT method. The concentrations of endothelin-1 and basic fibroblast growth factor in the supernatant were determined by ELISA. Results The proliferation of keratinocytes was promoted by 4.27, 5.49 and 6.1 mg/mL of the decoction, endothelin-1 secretion was stimulated by 1.83 and 5.49 mg/ml of the decoction in keratinocyte culture, but no effect on the secretion of basic fibroblast growth factor. Conclusions High concentrations of the decoction can promote the proliferation of keratinocytes. Different concentrations of the decoction can stimulate keratinocytes to secrete endothelin-1, which suggests that the decoction can stimulate keratinocytes to secrete endothelin-1 in vitro.更多还原