【摘要】 目的探讨与含c-Src同源序列SH2的酪氨酸磷酸酶(SHP-2)在尖锐湿疣组织中的表达及意义。方法采用免疫组化染色法检测了30例尖锐湿疣、13例宫颈癌和11例正常包皮组织中SHP-2的表达。结果包皮组织中磷酸酪氨酸和SHP-2阳性着色主要定位在表皮基底层角质形成细胞胞核,阳性表达细胞数量较少;而尖锐湿疣则相反,主要位于呈疣状增生浅层的凹空细胞胞浆内,阳性细胞成片分布;宫颈鳞状细胞癌的胞浆呈阳性表达,弥漫性分布,胞核呈阴性表达。尖锐湿疣和宫颈鳞状细胞癌中磷酸酪氨酸和SHP-2的阳性表达率分别为96.67%(29/30)和100%(13/13),而11例正常包皮均呈胞浆阴性表达。结论尖锐湿疣和宫颈癌细胞均存在与磷酸酪氨酸和SHP-2相关的细胞信息通路的异常激活。磷酸酪氨酸和SHP-2分布部位和阳性强度的改变可能与其癌变过程有关。更多还原

【Abstract】 Objective To explore the expression of tyrosine phosphatase containing c-Src homology SH2 (SHP-2) in condyloma acuminatum. Methods Phosphorylated tyrosine and SHP-2 were detected in skin lesions of 30 patients with condyloma acuminatum, tumor tissues of 13 cases of cervical cancer and normal foreskin tissues of 11 normal controls by SP immunohistochemical stain. Results In the foreskin tissue, the phosphorylated tyrosine and SHP-2 were mainly expressed on the nuclei of kerotinocytes adjacent to the basal layer, and the number of cells with positive expression was not so many. In condyloma acuminatum, the positive cells arranged in patches, phosphorylated tyrosine and SHP-2 distributed in the cytoplasm. In cervical cancer, the expression of phosphorylated tyrosine and SHP-2 were mainly in the cytoplasm, diffusely distributed, and not in the nuclei. The positive expression rates of phosphorylated tyrosine and SHP-2 in condyloma acuminatum and cervical cancer were 96.67% (29/30) and 100% (13/13), respectively. However the cytoplasm of normal foreskin showed negative expression(0/11). Conclusions There is an aberrant activation of phosphorylated tyrosine and SHP-2 in condyloma acuminatum and cervical cancer. SHP-2 may play an important role in the carcinogenesis.更多还原