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复痤霜中盐酸克林霉素和螺内酯的HPLC测定
Determination of Clindamycin Hydrochloride and Spironolactone in Compound Clindamycin Cream by HPLC
【摘要】 建立高效液相色谱法同时测定复痤霜中克林霉素和螺内酯的含量。采用C18柱,流动相为乙腈-磷酸盐缓冲液(pH7.4)(45∶55),检测波长214nm。克林霉素和螺内酯均在30~70靏/ml范围内线性关系良好(r>0.999),回收率大于98%,日内、日间RSD均小于2%。
【Abstract】 A HPLC method for simultaneous determination of clindamycin and spironolactone in compound clindamycincream was established. A C18 column was used with the mobile phase of acetonitrile-phosphate buffer solution(pH 7.4)(45∶55) at the detection wavelength of 214nm. The calibration curves of clindamycin and spironolactone were linear inthe concentration range of 30~70ìg/ml(r>0.999). The recoveries were more than 98%. The RSDs of intra- and inter-day were less than 2%.
【关键词】 盐酸克林霉素;
螺内酯;
乳膏剂;
高效液相色谱;
测定;
【Key words】 clindamycin hydrochloride; spironolactone; cream; HPLC; determination;
【Key words】 clindamycin hydrochloride; spironolactone; cream; HPLC; determination;
- 【文献出处】 中国医药工业杂志 ,Chinese Journal of Pharmaceuticals , 编辑部邮箱 ,2004年09期
- 【分类号】TQ463
- 【被引频次】6
- 【下载频次】54