节点文献
重组Pichia pastoris的发酵条件初步研究
Preliminary study on fermentation conditions of recombinant Pichia pastoris
【摘要】 在摇瓶中进行重组Pichiapastoris菌株SIBAS5071发酵条件的研究,考察了碳源、底物、pH、磷酸盐浓度、溶氧量等对该菌细胞生长和产生S-腺苷甲硫氨酸(SAM)的影响,初步确定的优化条件下发酵3d后,SAM产量可达2.3g/L(118mg/g干细胞)。
【Abstract】 The fermentation conditions of recombinant Pichia Pastoris SIBAS 5071 in the flake has beenperformed. Effects of fermentation conditions , such as carbon source, substrate, pH, concentration of phosphate buffer,dissolved oxygen on the cell growth and SAM production were considered. Under the optimized conditions, the produc-tion of SAM reached 2.3 g/L (118mg/g dry cell) after three days.
【关键词】 重组Pichia pastoris;
S-腺苷甲硫氨酸;
发酵条件;
【Key words】 S-adenosyl-L-methionine; recombinant Pichia pastoris; fermentation condition;
【Key words】 S-adenosyl-L-methionine; recombinant Pichia pastoris; fermentation condition;
- 【文献出处】 中国医药工业杂志 ,Chinese Journal of Pharmaceuticals , 编辑部邮箱 ,2004年02期
- 【分类号】TQ920
- 【被引频次】10
- 【下载频次】109