【摘要】 选择≤ 5岁 ,3 0～ 40岁 ,5 0～ 60岁 ,≥ 70岁四个年龄组的松质骨进行成骨细胞培养 ,第二代细胞培养至 14天用RT PCR检测骨保护蛋白 (OPG)、破骨细胞分化因子 (ODF)基因表达 ,结果显示不同年龄组人成骨细胞OPGmRNA、ODFmRNA表达量与年龄明显相关更多还原

【Abstract】 Human osteoblasts from donors of different age (≤5, 30-40, 50-60, ≥70) groups were isolated and cultured. Osteoprotegerin (OPG) and osteoclast differentiation factor (ODF) mRNA expressions were assayed by RT-PCR when the osteoblasts in the second passage were cultured for 14 days. Results showed that human osteoblasts from different age groups expressed OPG and ODF mRNA. Quantity of OPG mRNA and ODF mRNA expressions was correlated to donor′s age.更多还原