【摘要】 目的 研究四逆汤(SND)预先给药对大鼠肠缺血/再灌注(I/R)所致急性肺损伤的作用。方法 32只健康成年SD大鼠,雌雄不拘,随机分为4组:对照组(肠系膜上动脉仅分离而不阻断)、损伤组(肠系膜上动脉阻断1 h后再灌注3 h)、SND1 组(肠系膜上动脉阻断前3 d始连续经胃管灌入SND,总量为3 g/kg)、SND2组(肠系膜上动脉阻断前3d始连续经胃管灌入SND,总量为6 g/kg),每组8只。颈动脉插管监测平均动脉压(MAP)。每组动物均于再灌注3 h后断头处死。取肺组织测定肺通透性指数、肺组织一氧化氮(NO)、内皮素-1(ET-1)、丙二醛(MDA)的含量及超氧化物歧化酶(SOD)的活性,并在光镜下观察肺组织形态学的变化。结果 应用SND预先给药可明显减轻肠I/R引起的低血压和肺组织形态学改变。与对照组比较,损伤组肺通透性指数、肺含水率、肺组织MDA含量和NO含量显著性增高,而肺组织SOD活性显著性降低(P<0.01或0.05),两组肺组织ET-1含量无显著性差异。SND1组和SND2组肺组织SOD活性较损伤组显著性增高(P<0.01或0.05),但与对照组比较差异无显著性。结论 四逆汤预先给药通过抗氧化作用并减少NO的生成、维持NO/ET-1正常比例而减轻肠I/R引起的急性肺损伤。更多还原

【Abstract】 Objective To investigate the effects of pretreatment with Chinese herbal Sini Decoction ( SND) on the acute lung injury induced by intestinal ischemia / reperfusion (I/R) .Methods Thirty-two healthy SD rats of both sexes weighing 275-300 g were randomly divided into four groups of 8 animals : (Ⅰ) control group in which sham operation was performed, ( Ⅱ) I/R group in which superior mesenteric artery was clamped for 1 h followed by 3 h reperfusion; (Ⅲ) and (Ⅳ) SND group 1 and 2 in which SND 3 g·kg-1 ( Ⅲ) or 6 g·kg-1 (Ⅳ) was given via gastric tube every day for 3 days before I/R. Carotid artery was cannulated for MAP monitoring. The animals were sacrificed by decapitation at the end of 3 h reperfusion. Blood was collected and the lungs were immediately removed for determination of lung water content [ (wet weight - dry weight) / wet weight ×100% ], lung NO, endothelin-1 (ET-1) and MDA contents and SOD activity, lung permeability index (BALF protein concentration/serum protein concentration) and microscopic examination. Results SND pretreatment significantly alleviated the hypotension and morphological changes of the lungs induced by intestinal I/R. Lung water content, lung permeability index and lung MDA and NO contents increased significantly whereas lung SOD activity significantly decreased in I/R group ( group Ⅱ) compared with those in control group ( P < 0.01 or 0.05). There was no significant difference in lung ET-1 content between control and I/R group, resulting in increased NO/ET-1 ratio. SND pretreatment significantly prevented the above changes induced by intestinal I/R. Lung SOD activity was significantly higher in the two SND groups than that in I/R group but was still significantly lower than that in control group. Conclusion SND can attenuate the intestinal I/R induced lung injury by its antioxidant activity and by reducing the NO generation and maintaining normal NO/ET-1 ratio.更多还原