【摘要】 目的 探讨亚甲基四氢叶酸还原酶(MTHFR)677基因多态性与胃癌危险度的关系。方法 采用以人群为基础的病例对照研究方法,聚合酶链反应-限制性片段长度多态性法用于检测MTHFR677的基因型。结果 胃癌病例组中,MTHFR677 C/C、C/T、T/T三种基因型的频率分别为25.8％、54.6％、19.6％。对照组中三种基因型频率分别为34.5％、50.9％、14.6％。以C/C基因型作为参照基因型,C/T或T/T两种基因型的OR值为1.52(95％CI:1.04～2.23)。胃癌病例组中C和T两种等位基因型频率分别为53.3％和46.7％,对照组中分别为60.0％和40.0％。以C等位基因为参照,T等位基因的OR为1.31(95％CI:1.02～1.69)。此外研究还显示:MTHFR677AnyT基因型可能与吸烟、霉变粮食、大麦粥摄入、盐的过量摄入以及幽门螺杆菌CagA的感染发生交互作用而增加个体患胃癌的危险度。而该基因型与饮酒和饮茶未见明显的交互作用。结论 当个体携带C/T或T/T基因型时,患胃癌的危险度上升;携带AnyT基因型还可与其他环境危险因素发生交互作用,增加个体患胃癌的危险度。更多还原

【Abstract】 Objective To explore the relationship between methyl-tetra-hydroflic acid (MTHFR)677 gene polymorphism and the risk of stomach cancer. Methods A population based case-control studywas conducted and polymerase chain reaction-restriction fragment length poymorphism (PCR-RFLP)method was used to detect its genotypes. Results Among cases with stomach cancer, the frequency ofC/C, C/T, T/T genotype were 25. 8%, 54. 6%, 19. 6%, compared with controls as 34. 5%, 50. 9%,14. 6% respectively. Using C/C genotype as reference, the OR of C/T or T/T genotype was 1. 52(95%CI: 1. 04-2.23). 53. 3% C and 46. 7% T allele were distributed in stomach cancer cases, while 60. 0% Cand 40. 0% T in controls. The OR for T allele in relation to C allele was 1. 31 (1. 02-1.69) when C allelewas used as reference. In addition, the present study showed that MTHFR677 AnyT genotype mightinteract with smoking, moldy food intake, wheat porridge intake, eating salty food and Hp CagA infectionto increase the risk of stomach cancer. No interaction was observed between MTHFR677 AnyT genotypeand alcohol drinking or green tea intake. Conclusion MTHFR77 AnyT genotype, might increase therisk of stomach cancer development and the genotype might also interact with other environmental riskfactors to increase the risk of stomach cancer.更多还原