【摘要】 目的 监测肾综合征出血热(HFRS)疫苗免疫接种人群血清抗体水平变化并评价疫苗血清学效果。方法 以村为单位,16～60岁健康者(删除既往患HFRS者、外出>9个月及疫苗禁忌症者)为观察对象,按接种和对照各半的原则随机分组,接种组共10 460人,对照组16 159人。采用间接免疫荧光法测特异性IgG荧光抗体,用微量细胞病变中和试验测中和抗体。结果 接种前血清抗体阴性者全程接种后2周荧光抗体阳转率为100.0％(67/67,95％CI:96.3～100.0),中和抗体阳转率为44 4％(8/18,95％CI:22.0～69.0),几何平均滴度分别为72.1和4.6。1年后进行加强免疫,加强免疫前、免疫后2周,免疫后1年、1.5年、2年、3年和5年,荧光抗体阳性率分别为28.6％、83.3％、75.0％、53.1％、22.6％、10.0％、55.0％;中和抗体阳性率分别为14.8％、55.6％、35.0％、31.3％、26.0％、10.0％、50.0％。免疫(感染)增强反应需要进一步研究。结论 HFRS疫苗免疫效果良好,血清抗体持续时间较长。更多还原

【Abstract】 Objective To observe the serological and epidemiological efficacy of hemorrhagic feverrenal syndrome (HFRS) vaccine in Zhejiang province. Methods Immunofluorecent antibody assay andMcro-CPE method were used to test specific IgG antibody and the titer of neutralizing antibody. ResultsTwo weeks after the injection of the third dose, the sero-conversion rates by both immunofluorecentantibody test (IgG) and neutralization test were 100.0%(67/67)(95% CI: 96.3-100.0) and 44. 4%(8/18) (95% CI: 22. 0-69. 0) with geometric mean titers(GMTs) 72. 1 and 4. 6 respectively. The rates ofseroconversion of immunofluorescent antibody by immunofluorescence antibody assay (IFA) were 28. 6%,83. 3%, 75. 0%, 53. 1%,22. 6%, 10. 0% and 55. 0% before reinforcement, two weeks, one year, oneyear and a half years, two years, three years and five years after reinforcement. The rates of neutralizingantibody seroconversion by the Mcro-CPE method were found as 14. 8%, 55. 6%, 35. 0%, 31. 3%,26. 0%, 10. 0% and 50. 0% respectively. We found some antibody dependent immunization enhancementphenomenon among the inoculated population, but further observation was needed. Conclusion HFRSvaccine was immunologically effective and the duration of serous antithey last long.更多还原