【摘要】 [目的]探讨p53突变在脑胶质瘤发生发展中的作用及其与临床病理特征的关系。[方法]利用聚合酶链反应-单链构象多态性分析(PCR鄄SSCP)及LSAB免疫组化法对已确诊的48例脑胶质瘤进行p53基因突变以及蛋白表达的检测。[结果]48例胶质瘤中p53蛋白阳性表达率41.7%(20/48)。在高级别(Ⅲ、Ⅳ级)中p53表达率明显高于低级别(Ⅱ级)的肿瘤,分别是63.2%、27.6%(P<0.05)。PCR鄄SSCP检测发现17例(35.4%)呈现p53基因突变,均位于5～8外显子,突变例数依次为7(41.2%)、1(5.9%)、4(23.5%)、5(29.4%)。两种方法检测的符合率为89.6%(43/48)。p53基因的突变与患者性别、年龄、肿瘤部位及大小无关。[结论]胶质瘤中p53突变多在第5～8外显子,p53基因突变在胶质瘤的发生进展中起重要作用。更多还原

【Abstract】 To investigate the effect of p53 gene mutation in the pathogenesis of glioma and its correlation with clinicopathological features.p53 protein and p53 gene mutation were detected by LSAB immunohistochemistry and PCR-SSCP (single-strand conformation polymorphism) techniques in 48 cases of glioma. The positive rate of p53 protein was 41.7% (20/48) in 48 cases of glioma. The expression of p53 protein in high grade(Ⅲ,Ⅳ) glioma was significantly higher than that in the low grade(Ⅱ). Their expression rates were 63.2% ,27.6% respectively.Seventeen glioma cases with p53 mutations were detected by PCR-SSCP technique.The mutations located on 5~8 exon,7 cases(41.2%)exon 5;1(5.9%),exon 6; 4(23.5%), exon 7;5(29.4%),exon 8;respectively.The coincidence rate of immunohistochemistry and PCR-SSCP was 89.6%.p53 gene mutation was not associated with gender,age,tumor site and size. [Conclusion]The p53 mutation always locates on 5～8 exon and may play an important role in the pathogenesis in glioma.更多还原