【摘要】 目的 研究不同浓度苯并(a)芘(BaP)作用下A549细胞株热休克蛋白70家族(HSP70)多态性表达规律及其可能的意义。方法 利用二维聚丙烯酰胺凝胶电泳(2D-PAGE)技术分析A549细胞株中HSP70家族多态性表达。结果 A549细胞接受不同浓度BaP(0.1、1.0、5.0、10.0μmol/L)染毒24、48 h后,随着染毒剂量的逐步增加,HSP70家族中HSP72亚型的表达水平呈现逐渐下降的趋势(染毒24 h后积分光密度值分别为:150.36±26.03、98.57±1.3.34、64.92±15.03、34.65±19.10、32.92.±18.71;染毒48 h后分别为:126.85±17.41、106.19±15.32、73.64±21.02、35.18 ±11.95、16.27±9.35),而HSP73亚型的表达水平在不同浓度BaP染毒作用下无明显变化(染毒24 h后积分光密度值分别为:102.29±21.24、87.71±18.70、71.19±14.08、71.87±15.16、72.78±17.31;染毒48 h后分别为:86.66±16.86、75.67±10.61、66.83±12.63、67.29±10.26、91.37±13.68)。结论BaP对于HSP72表达有抑制作用,并且具有一定的剂量-效应关系,而对HSP73表达未见明显影响。更多还原

【Abstract】 Objective To study the pattern of polymorphism expression of heat-shock protein 70(HSP70) family in A549 cell line treated with different concentrations of benzo(a)pyrene(BaP) and its probable biological effect. Method Two-dimensional polyacrylimade gel electrophoresis(2D-PAGE) was used for the HSP70 expression analysis. Results 2D-PAGE showed that when A549 cells were exposed to different concentrations of BaP (0.1,1.0,5.0,10.0 μmol/L) for 24,48 h respectively,the HSP72 in A549 gradually declined as BaP concentrations increased[ the integral OD(IOD)] for 24 h were:150.36 ± 26.03,98.57 ± 13.34,64.92± 15.03,34.65 ± 19.10,32.92± 18.71 respectively, for 48 h:126.85± 17 .41,106.19 ± 15 .32,73 .64 ± 21.02,35 .18 ± 11.95, 16.27±9.35 respectively) , while the IOD of HSP73 did not show any remarkable change(24 h:102.29 ± 21.24, 87.71± 18.70,71.19 ± 14.08,71.87 ± 15. 16,72.78 ± 17. 31 respectively; 48 h:86.66 ± 16. 86,75.67 ± 10.61,66.83 ± 12.63,67.29 ± 10.26,91.37 ±13.68 respectively) . Conclusion BaP can inhibit HSP72 expression and with certain dose-effect relationship,but cannot affect HSP73 expression.更多还原