【摘要】 目的　探讨环氧化物水解酶 (mEH)的基因多态性和慢性苯中毒易感性的关系。方法　采用病例 -对照研究 ,以 15 2名苯中毒工人为病例组 ,15 2名接触苯而没有中毒表现的工人为对照组。应用多聚酶链反应 -限制性长度多态性分析技术 (PCR RFLP)检测mEH基因c .113和c.139位点的多态性。结果　携带mEHc .113C C基因型的个体发生苯中毒的危险性为携带有mEHc .113T T和T C基因型个体的 0 .6 0 (OR =0 .6 0 ,95 %CI:0 .37～ 0 .97,P =0 .0 4 ) ;在不吸烟的人群中 ,携带mEHc .113C C基因型的个体苯中毒的发病风险为携带T T和T C基因型的个体 0 .5 6 (OR =0 .5 6 ,95 %CI:0 .33～ 0 .96 ,P =0 .0 3) ;在不饮酒的人群中 ,携带有mEHc .113C C基因型又不饮酒的个体发生苯中毒的危险性为携带有mEHc .113T T和T C基因型的个体 0 .5 1(OR =0 .5 1,95 %CI:0 .30～ 0 .86 ,P =0 .0 1)。结论　携带有mEHc .113C C基因型 ,同时又不吸烟或不饮酒的个体发生慢性苯中毒危险性较低。更多还原

【Abstract】 Objective To explore the relationship between genetic polymorphisms of microsomal epoxide hydrolase(mEH) and susceptibility of chronic benzene poisoning(BP). Method A case-control study was conducted.152 BP patients and 152 workers occupationally exposed to benzene without poisoning manifestations were investigated.Polymerase chain reaction-restrained fragment length polymorphism technique(PCR-RFLP) was applied to detect the single nucleotide polymorphisms(SNPs) on c.113 and c.139 of mEH gene. Results The risk of BP for individuals carrying mEHc.113 C/C genotype was 0.60(OR=0.60,95%CI:0.37～0.97,P=0.04) of those carrying T/T and T/C genotypes.In non-smoking population,the risk of BP for subjects carrying mEHc.113 C/C genotype was 0.56(OR=0.56,95%CI:0.33～0.96,P=0.03) of those carrying T/T and T/C genotypes,and in non-drinking population,the individuals carrying mEHc.113 C/C genotype was 0.51(OR=0.51,95%CI:0.30～0.86,P=0.01) of those carrying T/T and T/C genotypes. Conclusion The subjects carrying mEHc.113 C/C genotype and together with non-smoking or non-drinking habit may have lower risk of chronic benaene poisoning.更多还原