【摘要】 目的　观察姜黄素对体外培养肝星状细胞 (HSC)增殖与凋亡的影响。方法　不同浓度姜黄素处理HSC株HSC T6 ,MTT法检测细胞增殖 ,流式细胞仪、透射电镜和琼脂糖凝胶电泳法检测细胞凋亡。结果　在 2 0～ 10 0 μmol/L浓度范围内 ,姜黄素可剂量依赖性地抑制HSC增殖 (P <0 .0 1)。 2 0、4 0、6 0 μmol/L姜黄素处理HSC 2 4h后 ,细胞周期分析发现S期细胞减少 ,G2 /M期细胞显著增加 (P <0 .0 1) ;流式细胞术检测到明显的亚G1峰 ,各组的凋亡指数 (% )分别是 15 .3± 1.9,2 6 .7± 2 .8,37.6± 4 .4 ,与对照组 (1.9± 0 .6 )相比 ,差异有显著性 (P <0 .0 1) ;4 0 μmol/L姜黄素作用 12、2 4、36、4 8h ,凋亡指数 (% )分别是 12 .0± 2 .4、2 6 .7± 3.5、33.8± 1.8和 4 9.3± 1.6 ,与对照组相比 ,差异有显著性 (P <0 .0 1) ;透射电镜观察到细胞皱缩、核染色质浓缩沿核膜排列和凋亡小体形成等 ;琼脂糖凝胶电泳可见到明显的DNA梯带形成。结论　姜黄素可显著抑制HSC增殖 ,使细胞周期停滞于G2 /M期 ,并诱导其凋亡 ,其作用具有时间和剂量依赖性更多还原

【Abstract】 Objective To investigate the effect of curcumin on proliferation and apoptosis of hepatic stellate cell line(HSC). Methods HSC line, HSC T6 was incubated with different concentration of curcumin. Cell proliferation was examined by MTT colormetric assay. Apoptosis was analyzed by flow cytometry(FCM),transmission electron microscopy(TEM)and agarose gel electrophoresis. Results Administration of 20 100 μmol/L curcumin could significantly inhibit HSC proliferation in dose dependent manner( P <0.01). After treated with curcumin at different concentration of 20,40,60 μmol/L for 24 h, the number of HSC in G2/M phase increased and in S phase decreased. There was significant sub G1 peak using FCM. Apoptosis indexes (%) were 15.3±1.9,26.7±2.8,37.6±4.4 respectively, which were significantly higher than that of control group(1.9±0.6, P <0.01). Incubated with curcumin for 12,24,36 and 48 h at the same concentration of 40 μmol/L, apoptosis indexes (%) were 12.0±2.4,26.7±3.5, 33.8 ±1.8 and 49.3±1.6 respectively, which were significantly higher than that of control group( P <0.01).Cell shrinkage, chromatin condensation and (or) ranked along inside of nuclear membrane and apoptosis body could be found by TEM. An oligonucleosomal DNA ladder of curcumin treated cells was demonstrated. Conclusions Curcumin significantly inhibits HSC proliferation, makes it arrest in G2/M phase and induces apoptosis of HSC, which effects are dose and time dependent.更多还原