【摘要】 目的 研究白细胞介素-10(IL-10)基因启动子区域-592、-819和-1082位单核苷酸多态性与自身免疫性肝病发病之间的关系。方法 分别采用聚合酶链反应-限制性片段长度多志性分析法(PCR-RFLP)和聚合酶链反应-序列特异性引物扩增法(PCR-SSP)检测54例自身免疫性肝炎(AIH)、77例原发性胆汁性肝硬化(PBC)患者及160例健康献血员外周血单个核细胞基因组DNA IL-10基因启动子区域3个多态位点-592、-819、-1082的基因多态性,并进行相关性分析。结果 54例AIH和77例PBC患者与160例健康对照组的IL-10启动子-1082、-819和-592基因型分布的统计学分析表明,差异无显著性。结论 IL-10启动子基因多态性与自身免疫性肝病发病之间无显著关联性。更多还原

【Abstract】 Objective To investigate whether three biallelic polymorphisms at the position-592,-819 and-1082 in the promoter region of the IL-10 gene were associated with the incidence of autoimmune liver disease. MethodsThe IL-10-592 and IL-10-1082 polymorphisms were genotyped by polymerase chain reaction-restriction fragmentlength polymorphisms analysis (PCR-RFLP), While polymerase chain reaction-sequence specific primer (PCR-SSP)assay was used to detect IL-10-819 polymorphisms. Result Among 54 Chinese patients with AIH or 77 Chinesepatients with PBC versus healthy controls, the frequency of AA, GA genotypes at IL-10 gene promoter-1082 positionwas 87.0% or 83.1% versus 90.0%, 13.0% or 16.9% versus 10.0%, respectively (P>0.05), the GG genotype in Chinesepopulations is absent; the frequency of CC, CT, TT genotypes at IL-10 gene promoter-819 position was 11.11% or 9. 1%versus 8.1%, 44.4% or 53.3% versus 45.0%, 44.4% or 37.7% versus 46.9%, respectively (P>0.05); the frequency of CC,CA, AA genotypes at IL-10 gene promoter-592 position was 4.9% or 14.3% versus 10.0%, 51.2% or 53.3% versus 51.9%,43.9% or 32.5% versus 38. 1%, respectively (P>0.05); No alleles differed significantly in each groups. ConclusionThere were no association between IL-10 gene polymorphisrns and autoimmune liver disease.更多还原