【摘要】 目的　探讨原癌基因C erbB 2蛋白、增殖细胞核抗原 (PCNA)、凋亡抑制基因 (Bcl 2 )、p5 3基因和转移抑制基因 (nm2 3 H1 )在乳腺癌患者接受简化根治术后几项指标同时检测对乳腺癌预后的指导作用。方法　对接受相同治疗方案 (改良根治术及术后放、化疗 )的 6 1例乳腺癌患者的术后石蜡切片标本进行免疫组织化学染色 ,检测C erbB 2、PCNA、Bcl 2、P5 3及nm2 3 H1 蛋白表达 ,并随访观察 5和 8年生存率。结果　不同的肿瘤大小、病理分级、淋巴结转移数 ,患者生存率差异有显著性 (P <0 0 5 )。雌激素受体 (ER)、孕激素受体 (PR)、C erbB 2、PCNA、Bcl 2、P5 3、nm2 3 H1 蛋白阳性及阴性表达组 5和 8年生存率差异均有显著性 (P <0 0 5 )。老年组和青壮年组生存率差异无显著性。结论　除传统的预后因素外 ,肿瘤细胞标志物 (C erbB 2、PCNA、Bcl 2、p5 3、nm2 3 H1 )均可作为判断乳腺癌预后的参考指标。更多还原

【Abstract】 Objective To examine the expressions of C-erbB-2,PCNA,Bcl-2,P53 and nm23-H₁ protein after modified radical mastectomy,and evaluate the relationship between their expressions and prognosis. Methods A total of 61 cases with breast cancer received the same treatment and their postoperative paraffin-embedded specimens were used for immunohistochemical study to examine the expressions of ER,PR,C-erbB-2,PCNA,Bcl-2,P53,nm23-H₁ protein. The 5,8-year survival rates were observed by fllow-up. Results The 5 and 8 year survival rates were statisticly different between the patients with different tumor size,histological grading,number of cancer metastasized lymph nodes （ P <0.05）.They were also statisticlly different between the patients with positive and negative expressions of ER,PR,C-erbB-2,and Bcl-2,PCNA,P53 and nm23-H₁ protein（ P <0.05）,but they were negatively correlated with patients’age. Conclusion C-erbB-2,Bcl-2,PCNA,p53 and nm23-H₁ gene can all become the independent prognostic factors of breast cancer.更多还原