【摘要】 目的　探讨小剂量放射治疗对血管弹性蛋白及弹性蛋白酶mRNA基因表达的影响。方法　以 0、7、14和 2 8Gy6 0 Coγ射线照射培养的大鼠主动脉平滑肌细胞 ,用逆转录 竞争性聚合酶链反应观察照射培养的大鼠主动脉平滑肌细胞弹性蛋白和弹性蛋白酶mRNA水平。结果　 7Gy照射对血管平滑肌细胞弹性蛋白和弹性蛋白酶mRNA的表达无影响 ,14和 2 8Gy照射后细胞弹性蛋白mRNA水平较对照组增加了 80 7%及 10 2 3% (P <0 0 5 ) ,细胞弹性蛋白酶mRNA水平较对照组减少了 2 5 3%及 5 0 1% (P <0 0 5 )。结论　提示 14和 2 8Gy6 0 Coγ射线照射通过抑制弹性蛋白酶及增加弹性蛋白合成而影响血管细胞外基质的代谢。更多还原

【Abstract】 Objective To study the effects of brachytherapy on the gene expessions of elastin and elastase in cultured rat vascular smooth muscle cells (VSMCs). Methods Rat VSMCs cultured in DMEM containing 10% FBS were irradiated by 60 Co γ-rays at 0,7,14,28 Gy respectively. Then mRNA levels of elastin and elastase were determined by reverse transcription competitive PCR(RT-PCR). Results Brachytherapy inhibited the expressions of elastase. Elastase mRNA decreased from 82.52±6.50 to 64.25±3.81 and 42.17±3.16 in VSMC irradiated with 14,25 Gy ,respectively( P <0.05). The elastin mRNA level increased from 95.17±6.41 to 72.05±5.82 and 49.77±5.62 in VSMC irradiated with 14,25 Gy ,respectively( P <0.05). Conclusion Brachytheapy inhabits the expressions of elastase and increased elastin in VSMC cells.更多还原