【摘要】 目的　探讨荧光原位杂交 (FISH)微核作为生物剂量计及评价职业受照射人群辐射效应的可行性。方法　用1 37Csγ射线不同剂量 (0 1～ 2 5Gy)照射离体人外周血细胞 ,用泛着丝粒探针的FISH技术进行微核分析 ,拟合剂量效应方程 ;检测医用X射线工作者的无着丝粒微核率并估算其生物剂量。结果　离体照射条件下微核率随吸收剂量增加而增加 ,以无着丝粒微核增加为主 ,着丝粒微核仅有轻度增加。微核率与剂量之间的拟合曲线方程 ,CB微核法 :Y =0 0 0 5 +0 0 36D +0 0 11D2 ,R2 =0 99;FISH微核法 :Y =0 0 0 1+0 0 35D +0 0 0 7D2 ,R2 =0 99。结论　FISH微核法优于CB微核法。用FISH微核法对医用X射线工作者的检测结果显示无着丝粒微核率可以用来估算生物剂量。更多还原

【Abstract】 Objective To investigate the feasibility of FISH micronucleus technique as a bio-dosimeter and evaluation the radiation effect of occupational population who exposed to radiation. Methods human peripheral blood samples were irradiated in vitro with 137 Cs γ rays with the doses ranging from 0.1 to 2.5 Gy,then CBMN was analyzed using FISH with Pancentromere DNA probe,and dose-response equation was estimated. And by detecting of the centromere-negative micronuclei rate of occupational exposure of the medical X-ray diagnostic workers in our country,the biological individual dose was estimated. Results Irradiatied in vitro,the micronucleus frequencies increased with the increase of radiation dose,and centromere-negative micronuclei accounted for the main increase of micronucleus and centromere-positive micronuclei only slightly increased. the dose-response relationships for CBMN assay and FISH assay were: Y_ CBMN = 0.0048 + 0.0362D + 0.0109D 2, R 2= 0.99; Y_ FISH = 0.0007+ 0.0353D +0.0070D 2,R 2= 0.99,respectively. Conclusions FISH micronucleus assay decreased the individual variation of spontaneous micronucleus,enhanced the radiation specificity of MN assay and was better than CBMN. The result shows that centromere-negative micronuclei rate can be used to estimate to biological dose.更多还原