【摘要】 目的　观察电离辐射对γ 氨基丁酸 (GABA)受体功能的影响以及动物受照射后神经行为的变化。方法　采用6 0 Coγ射线照射小鼠头部 ,观察不同剂量辐射对小鼠脑内神经细胞中GABA受体活性及其功能结合位点的影响 ,并对小鼠行为活动改变进行测定。结果　辐射可显著降低神经细胞中GABA受体生物结合活性 ,表现为GABA受体蛋白平衡解离常数 (Kd)升高 ,结合活性降低。受照射后GABA受体的异常变化与其受照射剂量有关。受照射小鼠行为测试结果显示 :照射 4 8h后 ,动物表现出燥动、焦虑等行为活动异常表现 ,与注射GABA受体阻断剂动物的行为活动变化相一致。结论　电离辐射可引起GABA受体活性改变。根据GABA受体阻断剂动物的行为变化实验结果 ,推测受照射后GABA受体功能和结合活性变化是引发神经行为变化的直接因素。更多还原

【Abstract】 Objective The purpose of this study was to investigate the effect of irradiation on gama-aminobutyric-acid receptor (GABA-R) as well as behavioral changes after brain 60 Co γ-irradiation. Methods The mice were irradiated with gamma rays (20 Gy;10 Gy and 5 Gy).The drug binding activity of GABA receptor in brain receptor was measured by fluorescence anisotropy (FA) and equilibrium dissociation constants.The behavioral changes were observed by the locomotor activity test,elevated plus-maze test and hole-board test at 1,10,24 and 48 hr after irradiation. Results 1.The drug binding activity of the GABA receptor was decreased and the equilibrium dissociation constant (K-d) was significantly increased compared with the negative control group 2 hr after irradiation,and a spike value appeared at 24 hr.It showed that the irradiation might damage or decrease the binding activity and the bio-activity of GABA receptor.2.The animal experiment confirmed that the irradiated animal model showed neural-behavioral changes of anxiety or depression.3.The decreased binding activity of GABA receptor and changes in behavior of irradiated animal were dependent on radiation intensity.4.The changes of behavior was similar to the blocked GABA receptor group.It suggests the relationship of radiation and GABA receptor. Conclusion These results suggest that GABA receptor may be involved in radiation injury.The functional changes of GABA receptor may be an induction factor of behavioral disorder.The article also discussed the effect of anxiety and results obtained from the point of view of GABA receptor system involvement in the changes observed after irradiation.更多还原