【摘要】 目的研究猪改良深低温动物模型深低温不停循环(DHNCA)90min期间脑保护作用机制。方法实验分二组假手术组6只,37℃不予特殊处理;实验组8只,18～25℃深低温不停循环90min,复温后观察生理指标变化、脑组织形态学和超微结构变化,用逆转录聚合酶链反应(RT-PCR)方法检测即早基因c-fos和c-junmRNA的表达。结果实验组光镜下可以观察到少量神经元形态学改变;电镜下线粒体形态基本正常,突触前膜有大量囊泡聚集;c-fos和c-junmRNA表达上调。结论猪改良动物模型在深低温脑保护研究中有效简便。深低温不停循环90min对神经组织无明显损害,可作为下一步深低温脑保护液辅助灌注实验的基础。更多还原

【Abstract】 Objective To study the role of the modified pig model with deep hypothermia not circulatory arrest (DHNCA) for 90 minutes in cerebral protection. Methods Pigs underwent cooling to 18℃ under modified cardiopulmonary bypass (CPB) technology without arrest. In sham operation group (Group A, n=6), CPB maintained in a state of normal temperature for 90 minutes. In experimental group (Group B, n=8), animals underwent 90 minutes of DHNCA at 18℃ 25℃.Following resume of temperature, an observation was done on the physiological changes, the morphological changes of the brain tissues and ultrastructural changes by photography and electron microscopy. In the meantime, the expressions of immediate early genes including c fos and c jun were examined. Results Slight morphological changes of nerons could be seen under photography microscope but morphology of nerons was normal under electron microscope that showed vesiculation in the presynaptic membrane in the Group B. RT PCR indicated the upregulated expressions of immediate early genes like c fos and c jun in the Group B. Conclusions The modified pig model with DHNCA is effective and simple in the study of cerebral protection. DHNCA for 90 minutes has no significant damage to neuron tissues, which may lay a foundation for the further experiment on the perfusion.更多还原