【摘要】 目的 :观察红花黄色素 (SY)的抗氧化作用。方法 :以邻二氮菲 Fe2 + 氧化法检测SY清除H2 O2 /Fe2 + 体系产生的羟自由基 ;硫代巴比妥酸比色法观察小鼠肝匀浆的脂质过氧化 ;比色法观察H2 O2 /Fe2 + 体系产生的羟自由基致红细胞膜的破裂。结果 :1.39～ 3.4 2g·L-1SY的羟自由基清除率为 2 7.2 %～ 10 0 % ,呈明显量效关系 ;77.8～776 .1mg·L-1SY缓解小鼠肝匀浆脂质过氧化的抑制率为 10 .5 %～ 92 .8% ,呈明显的量效关系 ;37.1～ 2 97.1mg·L-1SY抑制羟自由基引发的红细胞破裂呈明显的量效关系。结论 :SY为红花水溶性抗氧化有效部位。更多还原

【Abstract】 Objective: To observe the antioxidative effect of Safflor Yellow (SY). Method: Hydroxyl radical scavenge effect of SY was tested with 1,10-phenanthroline-Fe 2+ oxidative assay.Lipid peroxidation of mouse liver suspension was measured with thiobarbituric acid colorimetry technique. Hemocytocatheresis was determined with colorimetry. Result: Hydroxyl radical could be scavenged by 1.39 to 3.42 g·L -1 SY dose dependently. Mouse liver suspension peroxidation was inhibited by 77.8 to 776.1 mg·L -1 SY dosage dependently. Hemocytocatheresis was attenuated by 37.1 to 297.1 mg·L -1 SY dose dependently. Conclusion: SY is an antioxidative part of Carthamus tinctorius .更多还原