【摘要】 目的 :观察李氏 5号方在细胞水平上对神经细胞生长的影响 ,寻找最佳药物浓度。方法 :不同浓度的李氏 5号方作用于人神经母细胞瘤株SY5Y ,利用MTT测定细胞的存活率 ,制定浓度 药效曲线得出最佳药物浓度。以MTT代谢率、LDH漏出率、细胞轴突长度、胞体面积为观察指标。结果 :李氏 5号方在 0 .12 5～ 0 .75g·L- 1浓度范围内均可增加SY5Y细胞的存活率。与正常对照组相比 ,加药组SY5Y细胞轴突长度和胞体面积均增加 ,MTT代谢率升高、LDH漏出率降低。结论 :李氏 5号方具有营养神经细胞、促进神经细胞存活的作用更多还原

【Abstract】 Objective: To investigate the effect of Lishi No.5 formula on the growth of human neuroblastoma cell line SY5Y and find out the most effective drug dose. Method: SY5Y cells were administrated by Lishi No.5 formula in different concentration. MTT m etabolic rate was measured as the cell survival rate, then the dose-effect curv e was made. LDH leakage rate, axonal length and area of cell body were used as t he indicators. Reasult: In 0.125～0.75 g·L -1 Lish i No.5 formula could increase the cell survival rate and MTT metabolic rate, dec reased LDH leakage rate, and enhance axonal length and area of cell body, compa red with control group. Conclusion: Lishi No.5 formula ha s the neurotrophic effect and can induce the survival of neuron.更多还原