【摘要】 目的　探讨均匀双轴机械刺激对SD大鼠乳鼠成骨细胞环氧合酶 2 (cyclooxygenase 2 ,COX 2 )表达的影响。方法　设计了均匀双轴机械刺激模型 ,对成骨细胞给予不同时间 (2 ,4和 8h)和不同强度 (2 0 0 0 0microstrain ,10 0 0 0 0mincrostrain ,2 0 0 0 0 0microstrain)的机械刺激。利用免疫细胞化学和Westernblot检测均匀双轴机械刺激对成骨细胞COX 2表达的影响。结果　免疫细胞化学显示COX 2表达主要位于胞质 ,在核周聚集明显 ,Westernblot检测 2h机械刺激无显著增多 ,4和 8h机械刺激后COX 2的表达显著上调 ,但随刺激强度增加 4h的表达逐渐增多 ,8h的逐渐降低 ,与免疫细胞化学结果一致。结论　均匀双轴机械刺激在一定时间和强度范围内能促进大鼠乳鼠成骨细胞COX 2的表达。更多还原

【Abstract】 Objective To study the effects of uniform biaxial mechanical stimulation on cyclooxygenase (COX-2) expression in SD rat primary osteoblasts. Methods A new uniform biaxial mechanical stimulation model has been designed for the first time. The isolated rat primary osteoblasts were exposed to uniform biaxial mechanical stimulation at different intensities (20 000, 100 000 and 200 000 microstrains) for different time periods (2, 4 and 8 h). The expression changes of COX-2 protein caused by respective mechanical stimulation were detected by immunocytochemistry and Western blotting. Results The expression of COX-2 did not increase sigmificantly after mechanical stimulation for two hours, but was upregulated after four hour and eight hour stimulation, while in the control groups, nearly no COX-2 was expressed. In additon, the expression of COX-2 in four-hour stimulated group increased while in the eight-hour group decreased with mcreasing stimulation. Conclusion Uniform biaxial mechanical stimulation at a certain time period and intensity promotes the expression of COX-2 in rat primary osteoblasts.更多还原