【摘要】 目的:探讨人脑胶质瘤基质金属蛋白酶MMP2、MMP9和Ki-67的表达及其相关性。方法:应用MMP2、MMP9表达水平代表胶质瘤的侵袭活性,应用Ki-67标记指数代表胶质瘤的增殖活性;用免疫组化方法观察了6例正常脑组织、50例胶质瘤标本和2例恶性胶质瘤体外细胞系的MMP2、MMP9和Ki-67表达,并分析其相关性。结果:在胶质瘤中MMP2、MMP9的阳性表达率和Ki-67LI均随肿瘤恶性程度增加而增加并与胶质瘤分级呈正相关;相关分析发现,MMP2、MMP9和Ki-67LI的表达两两相关。结论:增殖和侵袭在恶性胶质瘤的分子生物学演进过程中相互协同、共同促进肿瘤细胞的恶性进展。更多还原

【Abstract】 Objective: To study the expression of Matrix Metalloproteinase(MMP)2, MMP9 and Ki-67 in human gliomas and the relationship between tumor proliferation and invasion. Methods: Tumor invasion was examed as the expression level of (MMP) 2 and MMP9. Proliferative activitiy of glioma was evaluated by Ki-67 Labling Index ( Ki-67 LI ) , and Immunohistochemical study was used for Ki-67, MMP2 and MMP9 expression in six normal brain tissue samples, fifty glioma samples and two glioblastoma cell lines. Results: The positive stained-cell rate of MMP2 and MMP9 increased with the degree of malignancy of tumors, and their differences of positive rate and expression level between the low-grade and high-grade tumors were also statistically significant. Ki-67 LI also increased correspondingly to the ascending of tumor grade, and the Ki-67 LI in grade III and IV was higher than that in low-grade gliomas. In addition, the expression of MMP2, MMP9 and Ki-67 correlated well with each other by Person correlation analysis. Conclusion: Proliferation and invasion cooperate with each other to promote the development of malignant phenotypes of gliomas.更多还原