【摘要】 目的:检测p14ARF、p16INK4a和p53蛋白在非小细胞肺癌中表达情况,对它们在非小细胞肺癌发生过程中的作用及其相互关系进行初步探讨。方法:应用免疫组化方法检测了40例非小细胞肺癌组织标本的p14ARF、p16INK4a和p53基因的表达水平。结果:非小细胞肺癌组织中p14ARF、p16INK4a和p53蛋白总阳性率分别是72.5%,50.0%和52.5%,与癌旁正常组织(分别是95.0%,92.5%,2.5%)有显著性差异(P<0.01);非小细胞肺癌中p14ARF和p53蛋白表达异常与肿瘤分期、组织类型、分化程度、淋巴结转移情况无显著相关性;p16INK4a蛋白表达水平与组织分化程度相关(P<0.05),但与肿瘤分期、组织类型、淋巴结转移情况无关;p14ARF和p53蛋白在组织中表达呈负相关,r2=-0.475(P<0.01),而p14ARF和p16INK4a蛋白表达无关;p14ARF蛋白在Ⅰ期病例中强阳性(+++)占阳性比例为41.7%,在Ⅲ期中强阳性(+++)比例为10.0%。结论:p14ARF、p16INK4a和p53三个基因在NSCLC发生过程中起重要作用,特别在肿瘤早期可能起到更为重要的作用;p14ARF和p53基因在同一调节通路上;p14ARF、p16INK4a和p53蛋白可以作为非小细胞肺癌早期生物学检测指标。更多还原

【Abstract】 Objective: To investigate the roles of p14ARF, p16INK4a and p53 gene in oncogenesis of non-small cell lung cancer(NSCLC ) and interrelations of them. Methods: The expression of p14ARF, p16INK4a and p53 gene was detected in NSCLC by immunohistochemistry analysis. Results: The positive rate of p14ARF, p16INK4a and p53 gene expression in NSCLC tissues was 72.5%, 50% and 52.5% respectively ,which was significantly different from that(95%, 92.5%, 2.5% respectively) in normal tissues adjacent to cancer (P<0.01). The expression level of p14ARF and p53 gene was not significantly related to histological classification, TNM stage, cancer cell differentiation and lymph node involvement in NSCLC. There was a significant relationship (P<0.05) between cancer cell differentiation and the expression level of p16INK4a which wasn’t significantly related to histological classification, TNM stage and lymph node involvement yet. A inverse significantly relationship was detected between p14ARF loss and p53 expression in the study (r2=-0.475, P<0.01), but there was not the significant correlation between p14ARF and p16INK4a expression. The overpositive proportion of all p14ARF positive cases in stage Ⅰwas 41.7% and 10% in stage Ⅲ. Conclusion: The aberrance of p14ARF, p16INK4a and p53 gene might be associated with the oncogenesis of non-small cell lung cancer. p14ARF and p53 gene might play roles in a same regulable pathway. The expression level of p14ARF, p16INK4a and p53 gene might be referred as markers of non-small cell lung cancer.更多还原