【摘要】 目的:观察DC与肿瘤细胞融合后的瘤苗体内诱导的抗肿瘤免疫应答以及对荷瘤小鼠的治疗作用。方法:应用免疫磁珠分选和贴壁培养方法收集融合细胞,应用3H-TdR掺入法、4h51Cr释放法观察T细胞增殖反应的能力和CTL活性,并观察瘤苗对荷瘤小鼠保护性免疫反应和免疫治疗作用。结果:DC肿瘤融合瘤苗具有强烈的激活T细胞增殖和抗原提呈的能力,在体外、体内诱导出更强的特异CTL细胞毒活性,使免疫小鼠产生一定的免疫保护作用,抵抗Hepa1-6肝癌细胞的再次攻击,使治疗的小鼠肿瘤的生长明显缓慢,具有更明显的治疗作用。结论:DC与肿瘤细胞融合后进行体内免疫和治疗,能诱导出显著的抗肿瘤免疫反应,为DC介导的肿瘤免疫治疗开辟了新的途径。更多还原

【Abstract】 Objective: To investigate the induction of antitumor immune response and therapeutic effects of the vaccination with the fused cells between tumor cells and dendritic cells. Methods: The fused cells were isolated by magnetic cell sorting and adherent culture, Proliferation of T lymphocytes and CTL activity was detected by 3H-TdR mix into assay and 4h 51Cr releasing assay. Results: DC cells-Hapal-6 cells fusion vaccines significantly increased the T cell proliferation, as well as the antigen prenting function of the tumor vaccine, and induced more potent and specific cytotoxic CTL activity in vivo and in vitro. After treatment with the fused cells, the tumor grew obviously slowly, and resisted against the rechallenge of Hepal-6 cells. Conclusion: Vaccination with the fused cells between tumor cells and dendritic cells could elicit potent antitumor responses, Which will provide a new approach to the DC-mediated therapeutic antitumor immunity.更多还原