【摘要】 研究了两种耐温耐碱的商业木聚糖酶酶活的影响因素:木聚糖酶A和木聚糖酶B酶反应的最适pH值为7.0,酶反应的最适温度分别为65℃和70℃。Fe³⁺在低浓时对这两种酶有激活作用,Fe³⁺在高浓时则有抑制作用。Al³⁺对木聚糖酶A有抑制作用,对木聚糖酶B有激活作用。Cu²⁺对木聚糖酶A和木聚糖酶B都表现为抑制作用。Mn²⁺对木聚精酶A有激活作用,对木聚精酶B有抑制作用。更多还原

【Abstract】 The influential factors of xylanases （EC3.2.1.8） activity were studied. Optimum pH for both xylanase A and xylanase B wns 7.0,the optimum temperature for xylanase A and xylanase B was 65℃ and 70℃ respectively. The activity of both A and B enzyme wasactivited by Fe³⁺ at low concentration, but inhibited by Fe³⁺ at high concentration. Al³⁺ had inhibition on xylanase A, but a promoting effecton xylanase B. The activity of both xylanase A and xylanase B was inhibited by Cu²⁺. Mn²⁺ activated xylanase A, but inhibited xylanase B.Xylanase A had K_m of 5.57 mg/mL and V_max of 1.98×10⁴μmol/ （mL· min ）. The K_m and V_max for xylanase B were 6.09 mg/mL and 3.64×10⁴μmol/ （mL·min）.更多还原