【摘要】 目的 :研究慢性肾功能衰竭 (CRF)患者血浆总同型半胱氨酸 (tHcy)水平及其与血清叶酸、维生素B12 (VitB12 )的关系。方法 :采用荧光偏振免疫分析法检测 4 5例CRF患者和 4 0例正常对照组血浆tHcy浓度 ,并同时用离子捕捉免疫分析法检测其血清叶酸和微粒子酶联免疫分析法检测血清VitB12 的含量。结果 :CRF患者血浆tHcy浓度 [(2 4± 12 ) μmol·L-1]显著高于正常对照组 [(8.0± 2 .9) μmol·L-1](P <0 .0 1) ;CRF患者血清叶酸水平 [(18± 6 .3)nmol·L-1]显著低于正常对照组 [(2 1± 8.3)nmol·L-1](P <0 .0 1) ,VitB12 水平 [(4 2 3± 15 4 )pmol·L-1]显著低于正常对照组 [(4 85± 181)pmol·L-1](P <0 .0 1)。相关分析显示 ,两组血浆tHcy浓度与叶酸、VitB12 水平均呈负相关性。结论 :CRF患者普遍存在高同型半胱氨酸血症 ,叶酸、VitB12 缺乏可能是诱发高同型半胱氨酸血症的重要因素之一。更多还原

【Abstract】 OBJECTIVE To investigate the plasma total homocysteine（tHcy） in the patients with chronic renal failure（CRF） and the relationship between plasma tHcy level and levels of serum folic acid and vitaminB ₁2（VitB ₁2）. METHODS Levels of plasma tHcy of 45 CRF patients and 40 normal subjects were determined using fluorescence polarization immunoassay（FPIA）,serum folic acid concentrations were measured by ion capture immunoassay（ICIA）,and serum VitB ₁2 concentrations were measured by microparticle enzyme immunoassay（MEIA）. RESULTS The mean level of tHcy in the CRF patients （24±12）μmol·L_-1 was significantly higher than that in the normal controls（₈.0±₂.9）μmol·L_-1（P<₀.01）. The mean level of serum folic acid in the CRF patients （18±₆.3）nmol·L_-1 was significantly lower than that in the normal controls（21±₈.3）nmol·L_-1 （P<₀.01）,and the level of serum VitB ₁2 in the CRF patients（423±154）pmol·L_-1 was significantly lower than that in the normal controls（485±181）pmol·L_-1 （P<₀.01）. Correlation analysis showed that plasma tHcy was negatively correlated with serum folic acid and VitB ₁2 in CRF group,and in normal group. CONCLUSION Hyperhomocysteinemia is more prevalent in the patients with CRF,deficiency of folic acid and VitB ₁2 is an important factor of inducing hyperhomocysteinemia.更多还原