【摘要】 菌株JMB142分别在NB、KB培养液中培养,发现其在KB培养液中生长较好。通过五个影响因子的正交试验分析了JMB142在KB培养液中的生长情况,并建立二次多项式回归模型。结果表明,JMB142在各个因素组合为温度33.2℃,起始pH值8.66,转速100r/min,培养时间40h,每250mL三角瓶通气量16.8mL时,孢子浓度最高达9.3×108CFU/mL;而且JMB142的最适培养温度、pH、转速、通气量、培养时间分别为32.6℃、7.8、148r/min、250mL三角瓶培养液40mL、61h。更多还原

【Abstract】 The tested isolate JMBb142 was incubated on KB and NB culture medium respectively. It was observed that JMB142 grew faster on KB culture medium than on NB. Orthogonal test for 5 several main factors affecting bacterial fermentation and growth on KB were arranged, and quadratic polynomial regression model was established. The results showed that in incubate conditions of temperature 33.2℃, pH 8.66, speed of agitation 100r/min, ventilated volume of 16.8mL/250mL-flask, time 40h, the spores concentration could reach as high as 9.3×108 CFU/mL. The optimum incubated temperature, pH, speed of agitation, ventilated volume and incubated time were 32.6℃, 7.8, 148r/min, 40mL/250mL-flask, 61h respectively?更多还原