【摘要】 目的 :考察几种冻干保护剂对载药SLN冻干过程中的保护作用 ,并筛选出最佳的配方。方法 :通过测定载药SLN冻干前后的载药量和粒度变化 ,来评价保护效果和优选配方 ,并通过TEM和AFM进行了形态学观察。结果 :浓度为 2 0 %的海藻糖可以对载药SLN起到较佳的保护效果 ,试样的包封率高达 79% (冻干前88% ) ,粒径 2 4 7nm(冻干前 10 9nm) ,并且多分散系数 0 .12 1大大低于冻干前的 (0 .2 4 6 ) ,为SLN载体系统的建立稳定剂型和长期保存开辟了前景。结论 :选用合适浓度的冻干保护剂在合理的冻干条件下可以获得令人满意的载药冻干SLN试样。更多还原

【Abstract】 AIM:Several cryoprotectants were employed to study the protective effect on the drug loading solid lipid nanoparticles(SLN) in order to select the optimum formulation.METHOD:The protective effect was evaluated by encapsulation efficiency and mean particle size of samples before and after freeze-drying process,and the morphologic study was performed by TEM and AFM.RESULT:The excellent protective effect could be obtained when trehalose was added to the sample with the concentration of 20%.Excellent sample was with the EE% of 79%(versus 88% of fresh one),mean particle size of 247 nm(versus 109 nm of fresh one),and a much lowered polydispersity index of 0.121(versus 0.246).CONCLUSION:The satisfactory result could be achieved with suitable type of cryoprotectant with appropriate concentration and proper freeze-drying condition.更多还原