【摘要】 用超声裂解、Sarkosyl处理和超速离心法提取禽大肠埃希氏菌O78强毒株 (简称O78)、O78耐氯霉素弱毒株 (简称O78r)、禽多杀性巴氏杆菌 5∶A强毒株C4 8 1(简称C4 8 1)、O78r 与C4 8 1的融合双价弱毒株F4 (简称F4 )菌体外膜蛋白 (OMP)。经SDS PAGE结果显示 ,O78、O78r、C4 8 1与F4 均有 1条分子质量为 31.0～ 4 3.0ku ,分子质量相近的OMP条带 ;O78与C4 8 1还有 1条分子质量小于31.0ku且相近的OMP条带。用OMP制备的油乳剂疫苗分别免疫小白鼠 15d和岭南黄鸡后 2 2d ,以O78、C4 8 1强毒菌攻击 ,结果表明O78、C4 8 1的OMP不但对同源菌具有免疫作用 ,且具有交叉免疫保护作用 ;用各自的OMP作包被抗原 ,建立的ELISA方法也能交叉检测到 3组OMP免疫鸡产生的抗 2种菌OMP的IgG抗体。更多还原

【Abstract】 The outer membrane proteins（OMP） of virulent avian Escherichia coli O₇₈ strain(O₇₈), virulent Pasteurella multocida C_48-1（5∶A） strain(C_48-1), attenuated avian Escherichia coli O₇₈^r strain(O₇₈^r ) and attenuated fusion strain of O₇₈^r-C_48-1(F₄ ), were extracted respectively by means of sonication, sarkosyl treatment and ultracentrifugation. The SDS-PAGE of the OMP show that one OMP band （about） 31 ku or 41 ku in molecular weight existed in all of the four strains. Another one OMP band with a molecular weight of 34 ku was found in O₇₈, O₇₈^r and C_48-1. Young mice and chickens were challenged by virulent O₇₈ and C_48-1 on day 15 and day 22 respectively after being vaccinated with the emulsified OMP of O₇₈ and C_48-1 or F₄. The results showed that all the OMP of the three bacteria have immunogenicity and can induce cross-protective immunity. The antibodyIgG of the chicken sera against all the three OMP can be detected with the OMP of O₇₈ and C_48-1as coated-antigens by ELISA.更多还原