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禽脑脊髓炎病毒VP3基因的克隆与序列测定

Cloning and sequencing of VP3 gene of avian encephalomyelitis virus

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【Author】 ZHANG Li-cheng~1, GUAN Yun-tao~2, YANG Zeng-qi~3, CHEN Hong-yan~2, WANG Yun-feng~2(1. Qinghai Provincial General Station of Animal Husbandry and Veterinary Medicine, Xining 810001,China; 2. Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001,China; 3. College of Animal Science and Technology, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100,China )

【机构】青海省畜牧兽医总站；中国农业科学院哈尔滨兽医研究所；西北农林科技大学动物科技学院；中国农业科学院哈尔滨兽医研究所青海西宁810001；黑龙江哈尔滨150001；陕西杨凌712100；黑龙江哈尔滨150001；

【摘要】根据已发表的禽脑脊髓炎病毒 (AEV)序列设计了 1对引物 ,利用RT PCR技术 ,从AEVVR株感染的SPF鸡胚脑以及内脏器官组织中成功扩增出了VP3基因 ,回收、纯化后克隆到T载体中 ,用常规方法转化JM1 0 9感受态细胞。阳性重组质粒经酶切及PCR鉴定后测定核酸序列。结果表明 ,VR株VP3基因全长 73 5bp ,共编码 2 4 5个氨基酸 ,与AEV 1 1 4 3标准毒株相应片段的核苷酸和氨基酸同源性分别为 93 .0 %和 97.0 % ;并将其与其他小RNA病毒进行了比较更多还原

【Abstract】 A pair of primers was designed according to the published avian encephalomyelitis virus (AEV) gene sequence, to amplify the van-roekel VP3 gene from the SPF embryo infected with AEV by the specific RT-PCR method. The amplified product was cloned into pMD 18-T vector after purification and withdraw. The recombinant plasmids were identified and sequenced. The result showed that the VP3 gene contained 735 bp nucleotides and encoded 245 amino acids which shared 93.0% and 97.0% homology with that of AEV-1143 strain respectively.更多还原

【关键词】禽脑脊髓炎病毒； VP3基因；克隆；测序；
【Key words】 avian encephalomyelitis virus； VP3 gene； cloning； sequence；

【文献出处】中国兽医科技 ,Chinese Journal of Veterinary Science and Technology , 编辑部邮箱 ,2004年11期

【分类号】S852.659.7
【被引频次】4
【下载频次】52

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