【摘要】 目的初步探讨甘草对大鼠小肠动力的作用,及其作用与胃肠激素的相关性。方法观察甘草组与空白组移行性综肌电(MMC)周期持续时间、Ⅲ相持续时间、Ⅲ相每分钟快波数(FM)和每簇的快波数(FC)的变化;采用免疫组织化学法结合显微图像定量分析扫描系统检测十二指肠、空肠嗜铬细胞及其肌间神经丛中5羟色胺(5HT)、P物质(SP)、血管活性肠肽(VIP)的相对含量。结果①甘草组与空白组比较MMCⅢ相FM和FC明显减少,MMC周期明显延长,Ⅲ相持续时间明显缩短,统计有显著性差异(P<0.05)。②甘草组十二指肠、空肠粘膜及肌间神经丛内5HT表达明显较空白组减少,比较有显著性差异,小肠粘膜无明显SP、VIP阳性免疫反映物表达,但小肠肌间神经丛内SP含量明显减少、VIP含量明显增加,组间比较有显著性差异(P<0.05,P<0.01)。结论甘草对大鼠小肠动力有抑制作用,这种抑制作用与5HT、SP、VIP分泌失调密切相关。更多还原

【Abstract】 Aim:To investigate the effects of glycyrrhiza decoction on migrating myoelectric complex(MMC)and gastrointestinal hormone in small intestine in rats.Methods:We observed MMC cycle,phase Ⅲ duration,fast wave numbers of phase Ⅲ of MMC in one minute, fast wave numbers of one cluster in phase Ⅲ of MMC of small intestine of glycyrrhiza group and control group rats with electrophysiology method,and immunohistochemistry to examine relative content of serotonin(5 HT),substance p(SP) and vasoactive intestinal polypeptide (VIP) in small intestinal chromophile(EC) and myenteric nerve plexus in small intestine of control group and glycyrrhiza group rats.Results:Compared glycyrrhiza group with control group,we found that glycyrrhiza was able to decrease fast wave numbers in one minute and fast wave numbers in one cluster in phase Ⅲ of MMC of small intestine( P <0.05),and evidently extend small intestinal cycle of MMC( P <0.05),it also shortened the phase Ⅲ duration ( P <0.05)or made the phase Ⅲ of MMC absent.Compared glycyrrhiza group with control group it was indicated that content of 5 HT in small intestinal mucous membrane and mytenteric nerve plexus was evidently decreased( P <0.05),and content of SP in mytenteric nerve plexus of small intestine of rats was evidently decreased ( P <0.05),and content of VIP in small intestine of rats was evidently increased( P <0.05). Conclusion:Cglycyrrhiza is able to inhibit small intestinal motility,this inhibition is related with the amount of 5 HT,SP,VIP secreted by small intestinal mucous membrane of rats.更多还原