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介绍一种简单、新型的主要用于蛙心灌注实验的计滴装置

A SIMPLE AND NEW TYPE OF DROP RECORDER MAINLY APPLIED IN THE EXPERIMENT OF FROG HEART

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【作者】 季淑梅王庆山范振中孙英

【Author】 JI Shu-mei,WANG Qing-shan,FAN Zhen-zhong,SUN ying (Department of Physiology,Hebei Medical University,Shijiazhuang 050017,china)

【机构】 河北医科大学生理室河北医科大学生理室 河北石家庄050017河北石家庄050017河北石家庄050017

【摘要】 目的 :介绍一种简单、新型的计滴装置的制作和使用方法。方法 :对蛙心灌流中过去曾使用过的ハ ム法和斯氏法进行了改进。取一个 2 0ml注射器 ,其内固定两个电极 ,用塑料管加工成出、入液管和阻力管。结果 :使用新型的计滴装置 ,可在离体蛙心观察前负荷、后负荷、激素和电解质对心功能的影响。结论 :经改进的计滴装置 ,制作经济 ,操作简单 ,和微机配合使用 ,更具直观性、准确性 ,能客观地反映各种理化因素对心功能的影响。

【Abstract】 Aim: To introduce the manufacture and use of a simple,new type of drop recorder of frog heart. Methods: To improve the perfusion device of ?-? and Straub method. Two electrodes of drop recorder were fixed in an injector of 20 ml. The input tube,output tube and resistance tube were all made of plastic material. Results: This device could be used to observe effects of preload,afterload,hormone and electrolyte on the cardiac output in isolated frog heart. Conclusion: The new type of drop recorder was economical and could be easily operated,it could be also connected to computer. Using the new type of drop recorder,effects of various physical and chemical factors on cardiac function could be observed directly,accurately.

【关键词】 蛙心脏心输出量前负荷后负荷
【Key words】 frog heartcardiac outputpreloadafterload
  • 【文献出处】 中国应用生理学杂志 ,Chinese Journal of Applied Physiology , 编辑部邮箱 ,2004年01期
  • 【分类号】R-331
  • 【被引频次】12
  • 【下载频次】149
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