【摘要】 目的 :探讨尾加压素II(UII)对于大鼠软脑膜微循环的影响。方法 :健康成年SD大鼠随机分为对照组、生理盐水 (NS)、UII(10 -7mol/L)、去甲肾上腺素 (NA ,10 -6mol/L)、UII(10 -7mol/L) +NA(10 -6mol/L)等五组 ,采用活体微循环观测技术观察大鼠软脑膜微血管内径、血流速度等微循环参数 ,采用激光多普勒血流量仪测定软脑膜血流量的变化。结果 :正常对照组软脑膜细动脉和细静脉血管内径分别为 (35 .4± 3.6 ) μm和 (40 .6± 8.5 ) μm ,UII组于滴加UII(10 -7mol/L)后即刻细动脉和细静脉出现收缩 ,1min时细动脉和细静脉收缩达到高峰 ,血管内径分别为 (2 5 .6± 3.4 ) μm和 (2 3.4± 3.3) μm (与正常对照组比较 ,P均 <0 .0 5 ) ;细动、静脉内血流速度无明显变化 (与正常对照组比较P均 >0 .0 5 ) ;软脑膜血流量于滴加UII(10 -7mol/L)后 1min开始升高 ,5min达到高峰 (3.5± 0 .4 )PU值 ,正常对照组 (2 .3± 0 .6 )PU值 (P <0 .0 5 )。结论 :UII可以使大鼠软脑膜微血管收缩 ,血流量增加。更多还原

【Abstract】 Aim: To investigate the effects of human urotensin II (hUII) on in vivo pia mater microcirculation in rats. Methods: Adult SD rats were randomly assigned to the following groups: control,sodium chloride injection(NS),UII(10 -6 mol/L),noradrenaline(NA,10 -6 mol/L),and UII (10 -6 mol/L)+NA(10 -6 mol/L) groups. For recording of microcirculation images in pia mater,skull windows were performed and mounted on the stage of an intravital microscope equipped with a TV camera. Video images of microcirculation were stored by a video cassette recorder. Temporal changes in internal diameter and microcirculatory velocity of microvesseles were measured by computer using the Image Pro software. The blood flow in cerebral tissues were measured with PIMII laser Doppler perfusion Imager (Lisca,Sweden). Results: The internal diameters of arterioles and venules in control group were (35.4±3.6)μm and (40.6±8.5)μm,respectively. In UII group,the arterioles and venules contracted immediately after treated with U II and up to the peak at 1 min,the internal diameters of arterioles and venules were (25.6±3.4)μm and (23.4±3.3)μm,respectively(P< 0.05 ). Both microcirculatory velocity in arterioles and venules had no significant changes in U II group(P>0.05). The blood flow in meninges increased 1 min after treated with U II and up to high peak at 5 min(3.5±0.4 perfusion unit vs. control 2.3±0.6,P<0.05). Conclusion: hUII can contract microvesseles in pia mater of rats and increase microcirculatory blood perfusion to cerebral tissue involved.更多还原