【摘要】 目的　了解温带地区早春猪舍三带喙库蚊自然感染流行性乙型脑炎病毒情况。方法　于 2 0 0 1和 2 0 0 2年 3月 1日～ 5月 31日 ,每日 18:0 0至次日 6 :0 0 ,对上海奉贤地区陈河洪猪舍采用诱蚊灯诱获蚊虫 ,应用逆转录一聚合酶联反应技术 (RT -PCR)进行乙脑病毒鉴定。结果　 2 0 0 1年共诱获成蚊 4 4 2 8只 ,2 0 0 2年 82 6 8只 ,2年蚊种鉴定结果一致 ,均分为 4属 8种。 2 0 0 1年和 2 0 0 2年的优势蚊种均为三带喙库蚊 ,构成比分别为 5 7 81%和 72 0 4 % ;吸血率比较有显著性差异 (χ2 =171 2 7,P <0 0 1)。 2 0 0 1年 5月 2 2日首先在三带喙库蚊检出乙脑病毒 ,检测 99批次标本 ,阳性率为 4 9 4 9% ;2 0 0 2年 5月 19日也是首先在三带喙库蚊检出乙脑病毒。结论　在温带地区蚊虫自然感染乙脑病毒于 5月中下旬开始 ,2年均以三带喙库蚊首先染毒 ,其带毒率明显高于其他蚊种 ,为预报流行性乙型脑炎的发生时间和流行强度提供科学依据更多还原

【Abstract】 Objective To investigate the natural infection of the epidemic encephalitis B virus of mosquitoes in the pig houses in early spring in the temperate zone.Methods From march 1 st to May 31 st ,every day from 18:00 p.m. to 6:00 a.m. of the next morning,mosquitoes were caught with lead-mosquito-lamp in the pig houses in Chenhebang,Fengxian district,Shanghai.The virus was identified with RT-PCT.Results 4?428 mosquitoes collected in 2001 and 8?268 in 2002 were identified of the same species,the 8 th species of the 4 th genus.The superior mosquito species caught in 2001 and those of 2002 were all Culex Tritaeniorhynchus ,constituting 57.81% and 72.04% respectively.There was remarkable difference in blood absorption between Culex Tritaeniorhynchus and other species of mosquitoes(χ 2=171 27, P <0 01).In Culex Tritaeniorhynchus caught in 2001,the encephalitis B virus was first found on May 22 nd .Of the 99 groups of samples examined,49 49% were male mosquitoes.In those caught in 2002,the virus was first identified on may 19 th .Conclusion The natural infection of encephaitis B virus of mosquitoes began in late May in the temperate zone. Culex Tritaeniorhynchus was the first infected species in both two years and was more likely to take the virus than other species of mosquitoes,which can provide scientific support for the prediction of the happening time and prevalence intension of the epidemic encephalitis B virus.更多还原