【摘要】 目的　探讨肠出血性大肠埃希菌 (EHEC)O157:H7在不同食品中的存活力。方法　采用EHECO157:H7933第 5代菌株人工污染 8种食品 ,染菌量为 7log10cfu/ml·g ;对该菌在 4、2 5℃条件下的存活力进行实验观察。 结果　EHECO157:H7在全奶、奶粉、鱼肉香肠及猪肉松 4种动物性食品中存活较长时间 ( 70d以上 ) ,且存活菌量呈先升后降的变化趋势 ,在 2种温度条件下均能增殖 1～ 2log10cfu/ (ml·g) ,到达高峰后呈对数衰减趋势 ,4 2d后仍有 3～ 6log10cfu/ml·g活菌量 ;在橙汁汽水和乳酸菌饮料中存活时间相对较短 ,分别为 2～ 5d和 10～ 2 7d ,4℃橙汁汽水和 2 5℃乳酸菌饮料在衰减至 4log10cfu/ (ml·g)后急剧下降 :在草莓酱和苹果酱中 ,两种温度条件下EHECO157:H7均呈间断性检出。结论　EHECO157:H7在常见食品中的存活能力强。加强食品特别是动物性食品及饮料的卫生管理是预防和控制该菌感染的重要措施更多还原

【Abstract】 Objective To investigate the survival of escherichia coli O 157 :H7 in eight kinds of food.Methods Eight kinds of food were collected and contaminated by the bacterial suspension of EHEC O 157:H 7 933 strain,the count of contaminated bacteria was 7 log 10?cfu/ml·g.Then the survival of E.coli O 157 :H 7 was observed experimentally at certain temperature (4,25℃) .Results E.coli O 157 :H 7 lived longer (>70 days) in animal food than other food.The trend of bacterial survival number rose firstly and then dropped.The bacteria both proliferated 1-2 log10?cfu/ml·g at two kinds of temperature and decreased logarithmically after reaching the peak.The bacterial count was still 3-6 log 10?cfu/ml·g after 42 days.The bacteria lived comparatively short (2-5 days in orange juice,10-27 days in lactic drink).The bacterial count rapidly decreased to 0 when it cut down on 4 log10?cfu /ml·g.E.coli O 157 :H 7 was found intermittently in both the strawberry jam and the apple jam.Conclusion E.coli O 157 :H 7 had strong survival in ordinary food.The major measure to prevent and control its infection was to strengthen the sanitary administration of food,especially animal food,and beverage.更多还原