【摘要】 目的　建立灵敏度高、简单方便、试剂安全的共振光散射方法测定生物体DNA含量。方法　在 pH5 5的HAc-NaAc缓冲体系中 ,将组蛋白与DNA混合 ,在入射光波长和荧光波长相同条件下 ,用普通荧光分光光度计测定溶液的共振光散射强度 ;光散射强度与DNA浓度呈良好线性关系时测定核酸含量 ;应用该法检测鼠肝和酵母体内的DNA含量。结果　扫描光谱显示最大光散射波长为 5 5 1nm ,测定最佳条件为pH5 5 ;组蛋白用量 10 μg/ml。ctD -NA、fsDNA、hpDNA等 3种核酸均与光散射强度有良好线性关系 ,相关系数均大于 0 99。与常规分光光度法对照测定样品DNA含量 ,相对误差 <10 %。结论　该方法灵敏度优于常规方法 2～ 3个数量级 ,快速方便 ,适合于生物体内核酸含量测定更多还原

【Abstract】 Objective To establish a sensitive,simple and safe method for the determination of nucleic acid in living body.Methods In the acetic acid-sodium acetate buffer solution at pH5.5,on the basis of the measarement of the light-scattering from a complex formed by DNA and histone using a common spectrofluorometer,the DNA in mouse liver and yeast was measured.Results The optimum measurement for DNA was at the wavelength of 551?nm;pH5.5;histon of 10?μg/ml.The linear relationship between the light-scattering intensity(I\-\{RLS\}) and the concentration of nucleic acids was good(r>0.99).The agreement between the light-scattering measurement and the conventional spectrophotometric method was satis-factory(relative error <10%).Conclusion The method was more sensitive than conventional spectrophotometic method,qaick,convenient and reliable with little interference.更多还原