【摘要】 目的　了解中国慢性乙型肝炎 (CHB)患者中的基因分型情况。方法　根据GenBank中 18株HBVS基因核苷酸序列 ,设计共同的外引物及其因型特异性内引物 ,对中国北方 5个城市 5 95例CHB患者血清进行基因分型 ,并对A、B、C及B、C混合型各一株进行测序。结果　 5 95例血清标本中 ,A型 8例 ,占 1 4 % ;B型 5 3例 ,占 8 9% ;C型 36 0例 ,占 6 0 5 % ;B、混合型 112例 ,占 18 8% ;A、C混合型 14例 ,占 2 4 % ;A、B混合型 15例 ,占 2 5 % ;A、B、C混合型 6例 ,占 1 0 % ;未分型者 2 7例 ,占 4 5 %。 5城市间在B、C及B、C混合型的分布上有显著性差异 (P <0 0 5 ) ,4份测序结果与PCR基因分型法一致。结论　在 5个城市CHB患者中HBV基因型以C型为主 ,B型次之 ,并见各种混合基因型感染 ,各地区在主要基因型分布上存在显著性差异。更多还原

【Abstract】 Objective To study hepatitis B virus (HBV) genotypes of patients with chronic hepatitis B in five cities of China.Methods 18 typical full-genomic sequences,including A,B,C,D,E and F genotypes of HBV were analyzed and aligned.Universal outer primers and type-specific inner primers were designed on the basis of conserved nucleotide sequences from pre-S1 to S region of HBV.595 serum samples collected from patients with chronic hepatites B in 5 cities of China were tested by the PCR genotyping method,4 samples genotyped by this method as A,B,C and coinfection of B and C were selected randomly and anlayzed by the cloning and sequencing.Results Of 595 serum samples,8(1 4%) were genotype A,53(8 9%) genotype B,360(60 5%) genotpye C 112(18 8%) were coinfection of genotype B and C 14(2 4%) A and C,15(2 5%) A and B 6(1 0%) A、B and C,and remaining 27(4 5%) unspecified.Distribution of gentype B、C and coinfection of genotype B and C between 5 cities was significantly different ( P <0 05).The results of sequencing were identical with that genotyped by the PCR method.Conclusion Genotype C was the most frequent genotype in patients with chronic hepatitis B in China,and genotype B was the next.Coinfections of two or more than two genotypes HBV were also found.Genotype distribution in patients with chronic hepatitis B in 5 cities studied was quite different.更多还原