【摘要】 目的 :检测用 9 cis RA处理前后肺腺癌细胞株PG和A54 9维甲酸受体RXRS 不同时相的表达变化。方法 :将两细胞株分别分为 9 cis RA组和对照组 ,应用细胞计数方法统计每组各时相细胞数 ,相对定量RT PCR方法检测组加药前后RXRS 各亚型在不同时相的表达变化。结果 :加入 9 cis RA后 ,PG和A54 9细胞RXRSmRNA转录水平检测结果显示 :(1)RXRγ 在两细胞株中均有一定水平的基础转录 ,而RXRα、RXRβ 均无基础转录。 (2 )应用 1uM 9 cis RA后 ,两细胞株的RXRγ 有短暂增高 ,其余受体转录无明显变化。结论 :RXRγ 可能参与介导 9 cis RA对两细胞株的诱导分化和凋亡作用更多还原

【Abstract】 Objective To detect expression changes of retinoid x receptor(RXR S)in different time-phases in PG and A549 lung glandular cancer cells before and after being treated with 1uM 9-cis-RArespectively.Methods Dividing PG and A549 lung glandular cancer cells each into two groups:9-cis-RA group and control group.Cell numbers of each group in different time-phases was by cell count methods.Reverse transcription polymerase chain reaction(RT-PCR) were used in study.Results The results that RT-PCR detected for the levels of RXR S transcription in PG and A 549 cells showed:(1)There was no basic transcription of RXRα and RXR β.(2)After being treated with 9-cis-RA,RXR γ increased in a short time.The rest receptors had no distinct changes in two cell lines.Conclusion 9-cis-RA can activate transcription of RXR γ mRNA in PG and A 549 cells.更多还原