【摘要】 目的　自制一种新型的高分子材料超声微泡造影剂 ,并观察其对新西兰大白兔的肾能量多普勒显像效果。方法　采用水 /油 /水 (water/oil/water,w/o/w)乳液 -溶剂蒸发、冷冻干燥法制备聚乳酸 /羟基乙酸 (poly lacticcoglycolic acid,PL GA)超声微泡造影剂 ,用扫描电镜观察微泡大小、形态 ,用血细胞计数仪测定微泡浓度。用生理盐水稀释粉末样 PL GA超声微泡造影剂至浓度为 10 8/ml左右 ,然后按 0 .2 ml/kg的剂量经兔耳缘静脉注射至兔体内 ,用能量多普勒观察其对兔肾显影的增强效果 ,同时监测兔基本生命体征变化。结果　PL GA超声微泡造影剂均一度高 ,平均粒径 3μm左右 ,最大直径 6 μm。与造影前相比 ,PL GA超声微泡造影剂能够明显增强兔肾能量多普勒显影效果 ,并且显像时间长。显像过程中未见兔基本生命体征有明显的变化。结论　 PL GA超声微泡造影剂能够明显增强兔肾能量多普勒显影效果 ,显像时间长 ,尚未发现明显的副作用 ,是一类具有广泛应用前景的新型超声造影剂。更多还原

【Abstract】 Objective To produce a kind of newly developed high molecular ultrasound contrast agent and to observe the energy Doppler imaging of new England rabbit kedneys Methods Polylacticoglycolic acid(PLGA)ultrasound contrast agents were produced using water/oil/water(w/o/w) and lyophilizing methods Electromicroscopic scanning and blood cells counter analysis were done to determine the bubble size,morphology and concentration The PLGA ultrasound contrast agent was diluted with normal saline to the concentration of 10 8/ml,and was injected into the vascular system through the ear vein of the rabbits Energy Doppler imaging was used to observe the effect of PLGA At the same time,the basic living signs of the rabbits were monitored Results The PLGA was highly uniformed and the average diameter was 3 μm and the largest was 6 μm They could greatly improve the quality of the energy Doppler imaging,and they lasted longer time than those of other contrast agents in vivo There were no abnormal changes of the rabbits’ basic life signs during the study,such as heart and respiratory rates,etc Conclusions The result of this study suggests that this novel high molecular ultrasound contrast agent is an effective and safe ultrasound contrast one in renal enhanced ultrasonography,and it is a great prospect of wide application.更多还原